Abstract: A chemiluminescent enzyme immunoassay method whereby a target substance such as a protein is assayed. This chemiluminescent enzyme immunoassay method comprises: the step of capturing an immune complex containing an enzyme-labeled antibody, which is labeled with an enzyme acting a chemiluminescent substrate, and the target substance on a support having no solution layer; the step of overlaying a support membrane containing the chemiluminescent substrate on the immune complex having been captured above; and the step of measuring the luminescence dose caused by the reaction between the enzyme-labeled antibody and the chemiluminescent substrate to thereby quantify the target substance.
Type:
Grant
Filed:
October 30, 2006
Date of Patent:
March 20, 2012
Assignees:
National University Corporation Hokkaido University, Seiken Co., Ltd., Aska Special Laboratory Co., Ltd.