Patents Assigned to ASM Scientific, Inc.
  • Methods For Multiplexing Recombinase Polymerase Amphlification
    Publication number: 20090269813
    Abstract: This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
    Type: Application
    Filed: July 25, 2006
    Publication date: October 29, 2009
    Applicant: ASM Scientific, Inc.
    Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes
  • Recombinase polymerase amplification
    Publication number: 20090017462
    Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
    Type: Application
    Filed: May 7, 2008
    Publication date: January 15, 2009
    Applicant: ASM Scientific, Inc.
    Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
  • 2'-Nitrobenzyl-Modified Ribonucleotides
    Publication number: 20080261208
    Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.
    Type: Application
    Filed: June 2, 2005
    Publication date: October 23, 2008
    Applicant: ASM Scientific, Inc
    Inventors: Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
  • Methods for multiplexing recombinase polymerase amplification
    Patent number: 7435561
    Abstract: This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
    Type: Grant
    Filed: July 25, 2006
    Date of Patent: October 14, 2008
    Assignee: ASM Scientific, Inc.
    Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes
  • Recombinase polymerase amplification
    Patent number: 7399590
    Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
    Type: Grant
    Filed: September 1, 2004
    Date of Patent: July 15, 2008
    Assignee: ASM Scientific, Inc.
    Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
  • Recombinase polymerase amplification
    Publication number: 20080076160
    Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.
    Type: Application
    Filed: August 13, 2007
    Publication date: March 27, 2008
    Applicant: ASM Scientific, Inc.
    Inventors: Niall Armes, Derek Stemple
  • Method for direct nucleic acid sequencing
    Patent number: 7270951
    Abstract: The present invention provides a novel sequencing apparatus and the methods employed to determine the nucleotide sequence of many single nucleic acid molecules simultaneously, in parallel. The methods and apparatus of the present invention offer a rapid, cost effective, high through-put method by which nucleic acid molecules from any source can be readily sequenced without the need for prior amplification of the sample or prior knowledge of any sequence information.
    Type: Grant
    Filed: March 10, 2000
    Date of Patent: September 18, 2007
    Assignee: ASM Scientific, Inc.
    Inventors: Derek L. Stemple, Niall A. Armes
  • Recombinase polymerase amplification
    Patent number: 7270981
    Abstract: This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods has the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods allow amplification of DNA up to hundreds of megabases in length.
    Type: Grant
    Filed: February 21, 2003
    Date of Patent: September 18, 2007
    Assignee: ASM Scientific, Inc.
    Inventors: Niall A. Armes, Derek L. Stemple