Abstract: The present invention provides a frontal light diffusing device comprising a non-circular core fiber section having a proximal end and a distal end, and a lens; wherein fiber core of the non-circular core section has a “top hat” core irradiance distribution and the frontal light diffusing device provides a “top hat” spatial irradiance distribution at a targeted location. The present invention further provides a frontal light diffusing device comprising an optical fiber and a collimation lens assembly wherein the collimation lens assembly includes a variable aperture that blocks portions of light outcoupled from the optical fiber thereby allowing only a central portion of the light to exit through the variable aperture resulting in a flat irradiance distribution.

Abstract: The present invention provides a cylindrical light diffusing device comprising a non-circular core fiber having (i) a fiber core that provides a “top hat” core irradiance distribution; (ii) light blocking means that prevent frontal light emisson from distal end of the non-circular core fiber; and (iii) a light diffusing section having a diffusing proximal end, a diffusing distal end, and internal scattering features distributed within the fiber core of the light diffusing section along central axis of the fiber core, wherein the light diffusion section emits irradiance in a radially symmetric longitudinally “top hat” diffusing irradiance distribution.

Abstract: Provided are methods for manufacturing a conjugate containing a phthalocyanine dye, including methods that include one or more steps of preparing or producing the conjugate, formulating the conjugate and packaging the conjugate. In some aspects, the manufacturing methods result in the generation of a stable conjugate. Also provided are stable phthalocyanine dye conjugates, compositions and articles of manufacture containing the stable conjugates, and methods for their administration to subjects for photoimmunotherapy. In some embodiments, the phthalocyanine dye conjugates are conjugated to a targeting molecule, such as an antibody, that targets the conjugate to a cell or pathogen, such as by binding to a cell surface protein.

Abstract: Provided herein are conjugates, compositions and methods for use in photoimmunotherapy, such as photoimmunotherapy induced by activation of a phthalocyanine dye conjugated to a targeting molecule that binds a protein on cell, for example, an IR700-antibody conjugate. In some embodiments, the phthalocyanine-dye conjugate can be activated by irradiation with near-infrared light. Features of the conjugates, compositions and methods, including the dose of the conjugate, provide various advantages, such as lower toxicity and/or improved efficacy. In some embodiments, also provided is a dual label phthalocyanine-dye conjugate in which the targeting molecule is conjugated to an additional fluorescent dye, which can be used for photoimmunotherapy while, for example, also exhibiting improved performance for imaging or detection. Also provided are therapeutic methods using the conjugates and compositions for treatment of diseases and conditions, including tumors or cancers.