Abstract: There are provided compounds and methods for amidating the C-terminus of a polypeptide. The method include reacting a polypeptide which includes a C-terminal thioester or C-terminal selenoester with any one of a defined set of auxiliary molecules under conditions suitable to produce a polypeptide adduct which includes the auxiliary molecule chemically bound at the C-terminal of the polypeptide. In the subsequent step, the auxiliary molecule is removed from the C-terminal of the polypeptide adduct under conditions suitable to produce an amidated polypeptide.
Type:
Application
Filed:
September 8, 2011
Publication date:
February 27, 2014
Applicant:
AstraZeneca Pharmaceuticala LP
Inventors:
Chengzao Sun, Behrouz Bruce Forood, Gary Luo, Soumitra S. Ghosh