Abstract: Disclosed are systems and methods for the cryopreservation of biological samples. The system including a mixing device configured hold the biological sample and a cryoprotectant and mix the contents therein. The mixing device can include a basket that moves within mixing device to ensure efficient mixing of the cryoprotectant. The system further includes a cooling device to cool the sample and cryoprotectant. The method includes placing a biological sample within the basket, such that the container is subject to cooling by the cooling device. Subsequently, cryoprotectant is added while the cooling device cools the biological sample. During this process the basket is moved within the container.

Abstract: The present invention relates to a method for freezing a water-containing quantity of a biological entity or a formulation in a vessel using a mineral nucleator, to the use of the mineral as a nucleator and to a vessel with the mineral in or on the whole or part of a surface thereof.