Abstract: Methods and systems for high-throughput sequencing data analysis are provided. In an embodiment, the method includes the following steps. An input DNA/RNA/Protein sequence is received by a master computing unit. The input DNA/RNA/Protein sequence is partitioned into overlapping segments with a sliding window less than a segment length of the overlapping segments to allow overlapping of any successive two thereof by the master computing unit. The overlapping segments are distributed by the master computing unit to a plurality of slave computing units in a cloud computing environment. Suffix-expansion-sorting processing is performed on the overlapping segments by the slave computing units to produce sorted expansion segments. Distributed database tables are generated based on the sorted expansion segments by at least a portion of the slave computing units.