Abstract: C. cohnii is cultured in a suitable growth medium with acetic acid/acetate as the main carbon source. The acetate is provided, and replenished, by adding acetic acid to the growth medium in response to an increase in pH resulting from the utilisation of acetic acid/acetate by C. cohnii. The C. cohnii produces relatively high levels of docosahexaenoic acid (DHA). A stationary phase is not essential for satisfactory DHA production.
Type:
Grant
Filed:
July 13, 2000
Date of Patent:
December 20, 2005
Assignees:
The University of Hull, ATV BV
Inventors:
Colin Ratledge, Alistair James Anderson, Kanagasooriyam Kanagachandran, David John Grantham, Janet Christine Stephenson, Martin E. de Swaaf, Lolke Sijtsma