Abstract: A method of recovering nucleic acid-containing particles from a liquid medium by contacting the liquid medium containing the particles with a mixture of hydroxylated silica beads and a salt solution to bind the nucleic acid-containing particles, centrifuging the mixture to pellet the bound particles, and separating the pellet from the liquid.
Abstract: An improved method for isolating and purifying nucleic acid from cell culture media contemplating adding to resuspended cell solutions a lysing solution and neutralizing/deproteinating agent without mechanical mixing, followed by centrifugation to partially pellet cellular debris, mixing the solution to complete the reaction, and additional centrifugation to fully pellet the debris.