Patents Assigned to Avellino Lab USA, Inc.
  • Publication number: 20240093319
    Abstract: The present invention provides compositions and methods for detecting and treating respiratory illness. The methods and compositions described herein comprise at least one probe that may hybridize with a target nucleotide molecule. The target nucleotide molecule may be a part of a virus, which in turn may be present in a subject, such as a human subject.
    Type: Application
    Filed: January 28, 2022
    Publication date: March 21, 2024
    Applicant: Avellino Lab USA, Inc.
    Inventors: Connie Chao-Shern, Jun-Heok Jang
  • Publication number: 20230348984
    Abstract: Systems and methods for detecting at least two genomic alleles associated with corneal dystrophy in a sample from a human subject are disclosed in which cells (e.g., epithelial) of the subject are adhered to a tip of a substrate. The tip of the substrate is agitated in a lysis solution that lyses cells adhered to the substrate. The substrate is removed from the lysis solution upon completion of this agitation. The resulting lysis solution is incubated and then genomic DNA from the lysis solution is isolated to form a gDNA solution. From this, identity of at least two nucleotides present in the human TGF?I gene is determined using at least two oligonucleotide primer pairs and the gDNA solution. These at least two nucleotides are located at respective independent positions of the TGF?I gene corresponding to respective independent single nucleotide polymorphisms (SNPs) associated with corneal dystrophy.
    Type: Application
    Filed: December 12, 2022
    Publication date: November 2, 2023
    Applicant: AVELLINO LAB USA, INC.
    Inventors: Connie Chao-Shern, Sun-Young Cho, Gene Lee
  • Patent number: 11525160
    Abstract: Systems and methods for detecting at least two genomic alleles associated with corneal dystrophy in a sample from a human subject are disclosed in which cells (e.g., epithelial) of the subject are adhered to a tip of a substrate. The tip of the substrate is agitated in a lysis solution that lyses cells adhered to the substrate. The substrate is removed from the lysis solution upon completion of this agitation. The resulting lysis solution is incubated and then genomic DNA from the lysis solution is isolated to form a gDNA solution. From this, identity of at least two nucleotides present in the human TGF?I gene is determined using at least two oligonucleotide primer pairs and the gDNA solution. These at least two nucleotides are located at respective independent positions of the TGF?I gene corresponding to respective independent single nucleotide polymorphisms (SNPs) associated with corneal dystrophy.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: December 13, 2022
    Assignee: Avellino Lab USA, Inc.
    Inventors: Connie Chao-Shern, Sun-Young Cho, Gene Lee
  • Publication number: 20210115499
    Abstract: The present disclosure relates to improved methods for the isolation of genomic material and detection of disease related single nucleotide polymorphisms. In some aspects, these methods increase the total recovery of genomic DNA from buccal cell samples by improving cell lysis conditions. In other aspects, these methods allow for the reuse of patient buccal swab samples, reducing the likelihood of having to collect additional patient samples for re-testing. Finally, in some aspects, these methods increase the sensitivity of SNP detection using an improved real-time PCR assay protocol.
    Type: Application
    Filed: December 28, 2020
    Publication date: April 22, 2021
    Applicant: Avellino Lab USA, Inc.
    Inventor: Connie Chao-Shern
  • Publication number: 20210032612
    Abstract: The present disclosure relates to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 9 (Cas9) systems, and methods of use thereof for gene editing or for preventing, ameliorating or treating a disease associated with a gene mutation or single-nucleotide polymorphism (SNP) in a subject.
    Type: Application
    Filed: February 22, 2019
    Publication date: February 4, 2021
    Applicant: Avellino Lab USA, Inc.
    Inventor: Tara Moore
  • Patent number: 10889850
    Abstract: The present disclosure relates to improved methods for the isolation of genomic material and detection of disease related single nucleotide polymorphisms. In some aspects, these methods increase the total recovery of genomic DNA from buccal cell samples by improving cell lysis conditions. In other aspects, these methods allow for the reuse of patient buccal swab samples, reducing the likelihood of having to collect additional patient samples for re-testing. Finally, in some aspects, these methods increase the sensitivity of SNP detection using an improved real-time PCR assay protocol.
    Type: Grant
    Filed: January 2, 2018
    Date of Patent: January 12, 2021
    Assignee: Avellino Lab USA, Inc.
    Inventor: Connie Chao-Shern
  • Publication number: 20200190587
    Abstract: Systems and methods for detecting single nucleotide polymorphisms (SNPs) associated with keratoconus (KC) in a sample from a subject are described.
    Type: Application
    Filed: April 27, 2018
    Publication date: June 18, 2020
    Applicant: Avellino Lab USA, Inc.
    Inventors: Larry DeDionisio, Tara Moore, Andrew Nesbit
  • Publication number: 20200040397
    Abstract: The present disclosure provides a method for detecting corneal dystrophy in a subject, comprising a reaction mixture, the reaction mixture comprising one or more labeled probes comprising a mutant TGFBI nucleotide sequence; the reaction mixture further comprises at least one amplification primer pair for amplifying a TGFBI gene sequence from a biological sample from the subject; and detecting one, two, three, four, five or six mutations selected from the group consisting of G623D, M502V, R124S, A546D, H572R, and H626R mutations in TGFBI gene, wherein the detecting comprises detecting the one or more mutations using the labeled detection probes. Further provided is a reaction kit comprising the reaction mixture.
    Type: Application
    Filed: April 10, 2018
    Publication date: February 6, 2020
    Applicant: Avellino Lab USA, Inc.
    Inventor: Connie Chao-Shern
  • Publication number: 20190185850
    Abstract: The present disclosure relates to single guide RNA (sgRNA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associate protein 9 (Cas9) system, and methods of use thereof for preventing, ameliorating or treating corneal dystrophies.
    Type: Application
    Filed: August 21, 2017
    Publication date: June 20, 2019
    Applicant: Avellino Lab USA, Inc.
    Inventors: Tara Moore, Andrew Nesbit, David Courtney, Katie Christie, Gene Lee
  • Publication number: 20190085288
    Abstract: Methods and compositions for the treatment of a corneal dystrophy in a subject in need thereof are provided. In one aspect, the method includes the step of obtaining a plurality of stem cells comprising a nucleic acid mutation in a corneal dystrophy target nucleic acid from the subject and manipulating the nucleic acid mutation in one or more stem cells of the plurality of stem cells to correct the nucleic acid mutation, thereby forming one or more manipulated stem cells. The manipulated stem cells are isolated and then transplanted into the subject. In some embodiments, the nucleic acid mutation is manipulated using CRISPR system.
    Type: Application
    Filed: May 11, 2018
    Publication date: March 21, 2019
    Applicant: Avellino Lab USA, Inc.
    Inventors: Tara Moore, Andrew Nesbit, Gene Lee, Sun-young Cho, Larry DeDionisio
  • Publication number: 20180230514
    Abstract: The present disclosure relates to improved methods for the isolation of genomic material and detection of disease related single nucleotide polymorphisms. In some aspects, these methods increase the total recovery of genomic DNA from buccal cell samples by improving cell lysis conditions. In other aspects, these methods allow for the reuse of patient buccal swab samples, reducing the likelihood of having to collect additional patient samples for re-testing. Finally, in some aspects, these methods increase the sensitivity of SNP detection using an improved real-time PCR assay protocol.
    Type: Application
    Filed: January 2, 2018
    Publication date: August 16, 2018
    Applicant: Avellino Lab USA, Inc.
    Inventor: Connie Chao-Shern
  • Patent number: 9856516
    Abstract: The present disclosure relates to improved methods for the isolation of genomic material and detection of disease related single nucleotide polymorphisms. In some aspects, these methods increase the total recovery of genomic DNA from buccal cell samples by improving cell lysis conditions. In other aspects, these methods allow for the reuse of patient buccal swab samples, reducing the likelihood of having to collect additional patient samples for re-testing. Finally, in some aspects, these methods increase the sensitivity of SNP detection using an improved real-time PCR assay protocol.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: January 2, 2018
    Assignee: AVELLINO LABS USA, INC.
    Inventor: Connie Chao-Shern