Abstract: The present invention provides a product comprising: a first component which is a scaffold; a second component which is an adjuvant, preferably a polypeptide which is a ligand for CD21 or a cell surface molecule on B cells or T cells or follicular dendritic or other antigen presenting cells; and a third component which is an antigen.
Type:
Application
Filed:
August 12, 2003
Publication date:
May 10, 2007
Applicant:
AVIDIS SA
Inventors:
Pierre Andreoletti, Laurence Dumon, Fergal Hill, Michel Julien, Jean Marchand, Emmanuel Risse
Abstract: The present invention provides a method for obtaining a recombinant fusion protein comprising a scaffold of a C-terminal core protein of C4bp alpha chain, said recombinant fusion protein being capable of forming multimers in soluble form in a prokaryotic host cell, the method including the steps of (i) providing a prokaryotic host cell carrying a nucleicacid encoding said recombinant protein operably linked to a promoter functional in said prokaryotic cell; (ii) culturing the host cell under conditions wherein said recombinant protein is expressed; and (iii) recovering the recombinant protein wherein said protein is recovered in multimeric form without performing a scaffold refolding step.
Type:
Application
Filed:
August 12, 2003
Publication date:
April 26, 2007
Applicant:
AVIDIS SA
Inventors:
Laurence Garnier, Fergal Hill, Michel Jullen