Abstract: The present invention provides an isolated protein comprising an immunoglobulin variable region comprising at least two cysteine residues positioned within framework region 1 such that if at least two of the cysteine residues are not conjugated to another compound a disulphide bond forms between the cysteine residues. Preferably the protein comprises an immunoglobulin heavy chain variable region and an immunoglobulin light chain variable region, wherein at least one of the variable regions comprises the two cysteine residues. The present invention also provides a protein that binds to TAG72. The present invention also provides conjugates of the protein and another compound.
Type:
Grant
Filed:
July 2, 2010
Date of Patent:
March 22, 2016
Assignee:
AVIPEP PTY LIMITED
Inventors:
Peter J. Hudson, Paul R. Sanders, Debra Tamvakis, Fabio Turatti, Michael P. Wheatcroft, David Leong
Abstract: The present invention provides an isolated protein comprising an immunoglobulin variable region comprising at least two cysteine residues positioned within framework region (FR) 2 and/or at least two cysteine residues positioned within framework region (FR3), wherein if at least two of the cysteine residues in FR2 and/or FR3 are not conjugated to a compound then an intra-framework disulphide bond is capable of forming between the cysteine residues. Preferably the protein comprises an immunoglobulin heavy chain variable region (VH) and an immunoglobulin light chain variable region (VL), wherein at least one of the variable regions comprises the two cysteine residues. The present invention also provides conjugates of the protein and another compound.
Type:
Application
Filed:
December 23, 2010
Publication date:
July 5, 2012
Applicant:
AVIPEP Pty Limited
Inventors:
Peter J. Hudson, Debra Tamvakis, Michael Paul Wheatcroft, Fabio Turatti