Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly lifts the injected eggs by causing a reduced pressure around the injection hole while maintaining the injection hole at atmospheric pressure. The injection assembly is sprayed with a sanitizing solution at the same time that the injected eggs are moved from the injection section to the transfer section.

Abstract: An in-ovo egg injection machine has a transversely or laterally movable supporting structure which is mounted beneath and adjacent the injector assembly and enables the operator to move egg trays into and out of the injection area from the side of the machine. The supporting structure includes two longitudinally extending opposed support rails that are mounted on a movable member so that the longitudinal support rails are spaced from one another at a set distance to define a support cradle. The movable member is supported on two transversely extending guide rails that are approximately twice the width of the injection area in length so as to allow the support cradle to be moved, preferably by hand, to an injection position directly under the injector assembly and then, by moving the support cradle in a lateral direction on the guide rails, to a loading/unloading position horizontally spaced from the injection area.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly lifts the injected eggs by causing a reduced pressure around the injection hole while maintaining the injection hole at atmospheric pressure. The injection assembly is sprayed with a sanitizing solution at the same time that the injected eggs are moved from the injection section to the transfer section.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly lifts the injected eggs by causing a reduced pressure around the injection hole while maintaining the injection hole at atmospheric pressure. The injection assembly is sprayed with a sanitizing solution at the same time that the injected eggs are moved from the injection section to the transfer section.

Abstract: An injection apparatus for injecting eggs of varying sizes and different orientations in an incubating tray that includes a substantially horizontal support plate with vertical openings therethrough, and vertical injectors resting in the openings and able to slide therein with respect to the plate such that the injectors can move vertically in the openings while remaining engaged with the support plate so as to maintain their vertical orientation throughout an injection sequence. For each opening, an expandable ring is positioned in the plate and pneumatically expanded to tighten around its respective injector after the injector has adjusted vertically in its respective opening to the height of an associated egg in order to maintain the injectors in their vertical orientation for injection of their associated eggs.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.