Abstract: Provided herein is a method to reduce the endotoxin contamination in plasmid preparations. In the described method, plasmid DNA and endotoxins are bound to an anionic exchange matrix and are brought into contact with a wash buffer, comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, wherein the branched secondary alcohol ethoxylates with the shorter ethoxylate chain is present in the washing buffer in excess compared to the branched secondary alcohol ethoxylate with the longer ethoxylate chain. The resulting purified plasmid has minimal endotoxin contamination levels and is considered endotoxin-free. Furthermore, provided are wash buffers comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, kits comprising such wash buffers and the use of such wash buffers for reducing the endotoxin contamination in plasmid preparations.

Abstract: The invention relates to an active substance-coated rod-shaped sample transfer tool, the preparation thereof, and the use thereof for transferring nucleic acid-containing sample material into reaction mixtures of enzymatic detection reactions.

Abstract: The invention relates to a process for isolating nucleic acids, such as DNA and RNA, using a non-alcoholic and non-chaotropic binding solution, and to the binding solutions and kits suitable for such a process.

Abstract: The invention comprises methods for isolating nucleic acids, such as DNA and RNA, while enzyme-inhibiting polyanions are reduced at the same time, using a non-alcoholic binder solution that is not based on chaotropic salts, and a washing solution containing an amine compound, and kits suitable for such a method, comprising the mentioned binder solution and washing solution.

Abstract: Provided herein is a method to reduce the endotoxin contamination in plasmid preparations. In the described method, plasmid DNA and endotoxins are bound to an anionic exchange matrix and are brought into contact with a wash buffer, comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, wherein the branched secondary alcohol ethoxylates with the shorter ethoxylate chain is present in the washing buffer in excess compared to the branched secondary alcohol ethoxylate with the longer ethoxylate chain. The resulting purified plasmid has minimal endotoxin contamination levels and is considered endotoxin-free. Furthermore, provided are wash buffers comprising a combination of branched secondary alcohol ethoxylates with varying ethylene oxide chain lengths, kits comprising such wash buffers and the use of such wash buffers for reducing the endotoxin contamination in plasmid preparations.

Abstract: Test device and method for the semi-quantitative determination of chlorine dioxide in a liquid sample has at least two carrier matrices. The first carrier matrix comprises at least one amino acid, sodium thiosulphate, at least one redox indicator, buffer substances and at least one surfactant. The second carrier matrix comprises at least one inorganic iodide salt, starch and/or at least one starch derivative, sodium thiosulfate, buffer substances and at least one surfactant.

Abstract: A method for treating a plurality of indicator fields for determining the pH-value of an aqueous solution is provided. Each of the indicator fields contains at least one indicator dye, which is fixed in a non-bleeding manner, in particular on a layer including cellulose or regenerated cellulose, and changes its color within a pH-turning-range. The indicator fields having buffer capacities which differ from one another. All the indicator fields are impregnated from the outside with an impregnation which influences the buffer capacities of the indicator fields in such a way that the deviations are levelled.

Abstract: The present invention relates to a device for determining the concentration of inorganic phosphate, especially orthophosphate, where said device is a test matrix including a molybdate salt, a solid acid, a PVP derivative, and a mixture of at least two different chromogenic reducing agents. In addition, the invention relates to a process for increasing the sensitivity of a test device.

Abstract: The invention comprises methods for isolating nucleic acids, such as DNA and RNA, while enzyme-inhibiting polyanions are reduced at the same time, using a non-alcoholic binder solution that is not based on chaotropic salts, and a washing solution containing an amine compound, and kits suitable for such a method, comprising the mentioned binder solution and washing solution.

Abstract: The present invention relates to a cellulose filter paper for testing the ignition propensity of cigarette paper that is provided with a marking with which the rough surface of the filter paper can be distinguished from the smooth surface, and a method for its production, a method for testing the ignition propensity of cigarette paper, and a kit having a plurality of such filter papers or stacks of such filter papers.

Abstract: The invention relates to a process for isolating nucleic acids, such as DNA and RNA, using a non-alcoholic and non-chaotropic binding solution, and to the binding solutions and kits suitable for such a process.

Abstract: The invention relates to an active substance-coated rod-shaped sample transfer tool, the preparation thereof, and the use thereof for transferring nucleic acid-containing sample material into reaction mixtures of enzymatic detection reactions.

Abstract: The invention relates to a device for extracting nucleic acid from a formalin-fixed and paraffin-embedded sample comprising a hollow body having an inlet port and an outlet port and a filter layer arranged within the hollow body, wherein the device is characterized in that the filter layer has an oil repellency of at least grade 5 according to the test method by AATCC-118 (1997). The invention further relates to a method for extracting nucleic acids from a formalin-fixed and paraffin-embedded sample, and a kit comprising the inventive device.

Abstract: Particulate solid composite material for purifying nucleic acids containing magnetic nanoparticles embedded in a carrier matrix based on at least one polymer that is obtained by polyaddition of a) at least one isocyanate-reactive monomer A, selected from compounds containing at least two functional groups, each having at least one Zerewitinoff-reactive hydrogen atom, and in addition to these at least two functional groups, carry at least one anionic or potentially anionic residue, preferably selected from the group consisting of carboxylate, sulfonate or combinations thereof, with b) at least one polyisocyanate monomer B with the provisio that said polyaddition occurs in the presence of magnetic nanoparticles, is particularly suitable as carrier material for purification of nucleic acids. Said composite material is easy to prepare, is stable, and magnetic and has outstanding application properties in the bind-wash-elute purification of nucleic acids using magnetic separation.

Abstract: Disclosed is a process for purifying nucleic acids, especially plasmid DNA, from a nucleic acid-containing, biologic sample, consisting of (a) preparation of a fluid sample containing nucleic acid and endotoxin each in dissolved form; (b) precipitation at least of nucleic acid and endotoxin from the fluid sample; (c) washing of the components of the fluid sample precipitated out in step b) in order to at least partially remove the endotoxin with at least one washing solution, which contains at least one amine compound with at least two carbon atoms and with a molar mass of ?500 g/mol and; at least one organic solvent different from the aforementioned amine compound and has a pH-value (20° C.) in the range from pH 3.0 to pH 8.5, and; (d) dissolution of the remaining nucleic acid from the washed, precipitated constituents from step c) using a dissolving buffer and collection of the dissolved nucleic acids in a separate receptacle. The process is suitable for the effective and economical removal of endotoxins.

Abstract: The subject innovation relates to a RNA binding buffer comprising (a) at least one chaotropic agent; and (b) an organic solvent selected from the group consisting of ethylene carbonate, ethylene glycol diacetate and 2-pyrrolidone or combinations thereof. The subject innovation further relates to a method of binding RNA to a solid support and to a method of isolating RNA both making use of the binding buffer of the subject innovation. The subject innovation finally relates to a kit comprising the RNA binding buffer or the organic solvent as relevant substance therein.

Abstract: A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.

Abstract: The subject innovation relates to a device for determining the concentration of a base and here especially hydrogen carbonate, whereby the device contains at least one pH indicator, at least one solid acid, and a mixture of at least one anionic surfactant and one nonionic surfactant. In addition, the subject innovation relates to a method for measuring the base concentration using the device according the subject innovation and a method for increasing the sensitivity of a testing device.

Abstract: The subject innovation relates to a RNA binding buffer comprising (a) at least one chaotropic agent; and (b) an organic solvent selected from the group consisting of ethylene carbonate, ethylene glycol diacetate and 2-pyrrolidone or combinations thereof. The subject innovation further relates to a method of binding RNA to a solid support and to a method of isolating RNA both making use of the binding buffer of the subject innovation. The subject innovation finally relates to a kit comprising the RNA binding buffer or the organic solvent as relevant substance therein.

Abstract: A device is provided for determining total chlorine in liquids, the device including an indicator carrier that is impregnated with a color indicator that contains Michler's thioketone, the indicator carrier additionally containing as a color indicator at least one azine as well as an iodide, in particular potassium iodide and/or sodium iodide.