Patents Assigned to AxioMx, Inc.
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Patent number: 11192939Abstract: The present invention provides methods and compositions for converting a first polypeptide into a chimeric polypeptide. The invention includes two vectors: a first vector including the sequence of the first polypeptide and a second vector including a second polypeptide. The vectors include complementary site-specific recombination motifs such that site-specific recombination between the two vectors results in the generation of a chimeric polypeptide including at least a portion of the first polypeptide and at least a portion of the second polypeptide. A site-specific recombination motif may be positioned within an intron or within a coding sequence on the first or second vector.Type: GrantFiled: April 11, 2018Date of Patent: December 7, 2021Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss, Melissa Batonick
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Patent number: 11078478Abstract: The identification of binding moieties capable of selectively interacting with one or more target antigens is of scientific, medical, and commercial value. Disclosed herein are methods and compositions for the identification, labeling and/or retrieval of cognate binding moieties.Type: GrantFiled: March 11, 2019Date of Patent: August 3, 2021Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Patent number: 11066662Abstract: Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as E. coli cells, yielding a library of variants. This method eliminates the inefficient sub-cloning steps and the need for costly primer sets required by many prior methods.Type: GrantFiled: April 12, 2019Date of Patent: July 20, 2021Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Patent number: 10301617Abstract: Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as E. coli cells, yielding a library of variants. This method eliminates the inefficient sub-cloning steps and the need for costly primer sets required by many prior methods.Type: GrantFiled: March 31, 2017Date of Patent: May 28, 2019Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Patent number: 10273472Abstract: The identification of binding moieties capable of selectively interacting with one or more target antigens is of scientific, medical, and commercial value. Disclosed herein are methods and compositions for the identification, labeling, and/or retrieval of cognate binding moieties.Type: GrantFiled: December 4, 2014Date of Patent: April 30, 2019Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Patent number: 10011655Abstract: Disclosed are methods of identifying binding moieties that recognize antigens displayed on cells, such as membrane proteins or recombinant proteins that display eptiopes on the surface of cells. Binding moieties capable of binding membrane proteins can be difficult to obtain because these proteins can depend on their native environments for structural integrity. In some methods scFv phage display libraries are panned against whole cells expressing a membrane protein in an emulsion. Certain methods further permit discrimination of binding moieties according to their affinity or avidity for a target. This approach allows rapid identification of cell surface epitope specific antibodies for research, diagnostics, and immunotherapeutics.Type: GrantFiled: December 19, 2013Date of Patent: July 3, 2018Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Patent number: 9969792Abstract: The present invention provides methods and compositions for converting a first polypeptide into a chimeric polypeptide. The invention includes two vectors: a first vector including the sequence of the first polypeptide and a second vector including a second polypeptide. The vectors include complementary site-specific recombination motifs such that site-specific recombination between the two vectors results in the generation of a chimeric polypeptide including at least a portion of the first polypeptide and at least a portion of the second polypeptide. A site-specific recombination motif may be positioned within an intron or within a coding sequence on the first or second vector.Type: GrantFiled: June 7, 2016Date of Patent: May 15, 2018Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss, Melissa Batonick
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Patent number: 9617537Abstract: Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution. In one embodiment, the method includes an amplification reaction, e.g., error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as E. coli cells, yielding a library of variants. This method eliminates the inefficient sub-cloning steps and the need for costly primer sets required by many prior methods.Type: GrantFiled: June 30, 2016Date of Patent: April 11, 2017Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss
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Publication number: 20170029811Abstract: The identification of binding moieties capable of selectively interacting with one or more target antigens is of scientific, medical, and commercial value. Disclosed herein are methods and compositions for the identification, labeling, and/or retrieval of cognate binding moieties.Type: ApplicationFiled: December 4, 2014Publication date: February 2, 2017Applicant: AxioMx, Inc.Inventors: Michael WEINER, Margaret KISS
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Patent number: 9422549Abstract: Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as E. coli cells, yielding a library of variants. This method eliminates the inefficient sub-cloning steps and the need for costly primer sets required by many prior methods.Type: GrantFiled: February 26, 2014Date of Patent: August 23, 2016Assignee: AxioMx, Inc.Inventors: Michael Weiner, Margaret Kiss