Abstract: The present disclosure generally relates to a cell culturing system, and specifically to a three-dimensional cell culturing system for neuronal cells that promotes both structural and functional characteristics that mimic those of in vivo peripheral fibers, including cell myelination. Using a dual hydrogel construct and spheroids comprising neuronal cells, the present disclosure provides methods, devices, and systems for in vitro spatially-controlled, three-dimensional models that permit intra- and extra-cellular electro-physiological measurements and recordings. The three-dimensional hydrogel constructs allow for flexibility in incorporated cell types, geometric fabrication, and electrical manipulation, providing viable systems for culture, perturbation, and testing of biomimetic neural growth with physiologically-relevant results.
Type:
Application
Filed:
December 4, 2018
Publication date:
December 10, 2020
Applicants:
The Administrators of the Tulane Educational Fund, AxoSim Technologies LLC
Inventors:
Michael James Moore, Jabe Lowry Curley, Anup D. Sharma, Devon Bowser, Jordan Behn