Abstract: Provided herein is a real-time PCR-based method of detecting, in a sample, an agent causing onychodystrophy, wherein the agent causing onychodystrophy belongs to a secondary clade member including one or more primary clade members. Also provided are compositions and kits that finds use in implementing the present method.
Type:
Grant
Filed:
February 20, 2020
Date of Patent:
January 3, 2023
Assignee:
BAKOTIC PATHOLOGY ASSOCIATES, LLC
Inventors:
Idowu Akinsanmi, Lori Bennett, Liquan Yang, Marianna Agassandian, Rama Murthy Sakamuri, Erik Gustafson, Lilly Kong, Kiran Madanahally Divakar
Abstract: Provided herein is a method of detecting an onychomycotic fungus in a sample, wherein the onychomycotic fungus belongs to a secondary clade member including one or more primary clade members. The method may include the steps of i) screening a sample using a first and second sets of secondary clade-specific primers to determine whether a secondary clade member among a plurality of secondary clade members is present or absent in the sample, where the plurality of secondary clade members includes (a) a dermatophyte, (b) a candida, and (c) a saprophyte, and ii) after determining the presence of the secondary clade member, screening the sample to determine whether an onychomycotic fungus is present or absent in the sample using primary clade-specific primers that are specific to a primary clade member that belongs to the secondary clade member. Also provided is a kit that finds use in implementing the present method.
Type:
Grant
Filed:
July 28, 2016
Date of Patent:
August 16, 2022
Assignee:
Bakotic Pathology Associates, LLC
Inventors:
Kiran Madanahally Divakar, Bradley Wayne Bakotic, Laurie Susan Page, Lori Ilene Bennett