Abstract: Methods and systems for diagnosis, detection, monitoring, and treatment of traumatic brain injury are described. The methods and systems include detection of salivary biomarkers associated with brain injury in a human subject, one application of which is to determine whether the subject has sustained a concussion or a more severe traumatic brain injury (TBI). Detection of the salivary biomarkers can also provide a basis to determine that a subject can safely return to play in an athletic event and can provide a basis to evaluate the efficacy of particular treatments. The methods and systems may be implemented, for example, by means of a kit.

Abstract: Assay devices, systems, methods, and kits useful for detection of analytes in a bodily fluid sample, such as saliva, are disclosed herein. Assay devices may include a fluid sample collector and an assessment unit to which the fluid sample collector couples. The fluid sample collector may include a cap with a plunger and absorbent member that collects a bodily fluid sample. Insertion of the plunger into the assessment unit during coupling provides pressure-driven fluid flow through a filter rapidly delivering bodily fluid filtrate to one or more test panels which provide the assay result. In embodiments, an on-board light source enables a user to readily detect changes in a test panel indicative of the presence of an analyte thus providing an opportunity for obtaining rapid results with improved confidence.