Abstract: The present invention pertains to the field of imparting or increasing resistance against fungal pathogens, in particular soy-beanrust, in plants, plant parts, and/or plant cells. This is achieved by increasing the content of scopoletin, scopolin and/or, most preferably, scoparone (6,7-di-methoxycoumarin) in a plant, plant part and/or plant cell. This can in particular be achieved by establishing and/or increasing the expression of an OMT3 enzyme and an F6H1 protein in the respective plant, plant part and/or plant cell; a further way would be to apply a formulation or solution containing scopoletin and/or, most preferably, scoparone to the plant, plant part and/or plant cell. The invention correspondingly also provides proteins, enzymes, expression constructs, plants, plant parts and/or plant cells. In this respect the invention also provides variants of the OMT3 enzyme.

Abstract: The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention, wherein the buffer has a strong alkaline pH of 10 to 12.5 and comprises a soluble concentration of detergent at a level of 0.5% to 5%.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.

Abstract: Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13° C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.

Abstract: The present invention relates to a process for the production of polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which encode polypeptides with ?5-elongase activity. Advantageously, these nucleic acids can be expressed in the organism together with further nucleic acids which encode polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Especially advantageous are nucleic acids which encode ?6-desaturases, ?5-desaturases, ?4-desaturases and/or ?6-elongases. These desaturases and elongases are advantageously derived from Thalassiosira, Euglena or Ostreococcus. The invention furthermore relates to a process for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids, and oils and/or triacylglycerides thus obtained.

Abstract: The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, ear fresh weight, and single kernel weight, and increased yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more molybdenum cofactor (Moco) biosynthesis polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present invention provides transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.

Abstract: The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK1protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK1protein.

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing constitutive promoters and the production of plants with enhanced constitutive expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to the promoters and/or introduced into plants.

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK2 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK2 protein.

Abstract: The present invention is concerned with the provision of means and methods for gene expression. Specifically, it relates to a polynucleotide comprising an expression control sequence which allows for seed specific of a nucleic acid of interest being operatively linked thereto in plants. Furthermore, vectors, host cells, transgenic plants and methods for expressing nucleic acids of interest are provided which are based on the said polynucleotide.

Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

Abstract: Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.

Abstract: The present invention relates to the field of producing particular metabolites of interest by engineered crop plants such as transgenic crop plants. Provided are methods that are easily applicable by farmers to determine when the metabolites of interest hake reached an optimal content in the plant. These methods also help to facilitate decisions about the timeframe for preparing harvest or harvesting the engineered crop plant.

Abstract: Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.