Abstract: An injection site and unsharpened drug transfer spike for facilitating coupling a syringe to a fluid line. The injection site has a uniquely designed housing and septum comprised of only two parts. The drug transfer spike has a positive locking member for restricting retraction from the injection site once it is inserted for injecting a medicant. The positive locking member preferably comprises a annular ridge defined about the shaft of the drug transfer spike. The septum includes a pair of axially aligned, oppositely oriented conical recesses with adjacent apices either separated by a thin membrane or intersecting at the time of manufacture. The housing has an inlet for receiving the septum and comprises a plurality of fingers with inwardly extending tabs for constraining the septum therewithin. The septum is ideally adapted to be applied to injection sites, Y-connectors, vial adaptors, single or multi-dose vials and blood collection tubes.