Abstract: Vectors which establish controlled expression of recombinant GHRH genes within tissues at certain levels. The vector includes a 5′ flanking region which includes necessary sequences for expression of a nucleic acid cassette, a 3′ flanking region including a 3′ UTR and/or 3′ NCR, and a linker which connects the 5′ flanking region to a nucleic acid sequence. The linker has a position for inserting a nucleic acid cassette. The linker does not contain the coding sequence of a gene that the linker is naturally associated with. The 3′ flanking region is 3′ to the position for inserting the nucleic acid cassette.
Type:
Application
Filed:
April 16, 2002
Publication date:
March 20, 2003
Applicant:
Baylor College of Medicine and GeneMedicine
Inventors:
Robert J. Schwartz, Ruxandra Draghia-Akli, Xuyang Li, Eric M. Eastman