Abstract: A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.

Abstract: A holder for consumables for use in an analyzer is disclosed. The holder has one or more receptacles for receiving a stack of consumables. The consumables are wrapped in packaging and are placed in the holder as a stack of packaged consumables. The receptacles have a height sufficient to receive the stack of consumable. The receptacles have an opening miming along the side of the receptacle for the entire portion of the height of the receptacle that receives the stack of consumables. The receptacles have a proximal end and a distal end. The proximal end is the end of the receptacles into which the stack of consumables is place and from which the consumables are removed. The distal portion of the receptacles support the stack of consumables in the receptacles.

Abstract: The present invention describes an automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.

Abstract: An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.

Abstract: An automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/‘order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis.

Abstract: A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior.

Abstract: An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.

Abstract: A blood metering device for determining an accurate target fill volume of blood in a collection vessel is disclosed herein. The blood metering device includes a housing having an inlet and an outlet, a blood flow conduit defined in the housing for providing a continuous conduit from the inlet to the outlet, and a valve disposed in the blood flow conduit. A valve operation is controlled by a valve actuator for moving the valve from the open position to the closed position. The valve actuator is responsive to a measured gas pressure in a collection vessel in fluid communication with the outlet of the housing where the valve is moved to the closed position when the measured gas pressure is approximately equal to a target gas pressure. A method for determining an accurate target fill volume of blood in a collection vessel is also disclosed herein.

Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.

Abstract: An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.

Abstract: An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.

Abstract: An improved system and method for the selection and dispensing of reagents onto a target testing medium in an automated laboratory environment. The system utilizes multiple carousels rotatably-mounted upon a central turntable. The position of the central turntable, as well as the rotation of each carousel is controlled by a microprocessor-based control system. Multiple dispensers, each capable of storing and dispensing a particular reagent, are mounted about the circumference of each carousel. This configuration provides much higher reagent density than previously available. The testing medium is positioned at one or more loading stations. The control system directs the central turntable to a position from which a selected one of the carousels can be rotated so as to position a selected dispenser above the testing medium. The selected dispenser is then actuated to release a predetermined reagent onto the testing medium.

Abstract: A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior.

Abstract: A system that may automatically and rapidly process blood culture bottles. Such processing includes obtaining an image of a label placed on the cylindrical surface of the blood culture bottle. The system may also determine an amount of blood sample that has been added to the blood culture bottle by comparing a sample level to pre-placed fiducial. The distance between the liquid meniscus in the blood culture bottle and fiducial may be used to determine blood volume. The imaging apparatus may also detect internal conditions of the blood culture bottle such as the presence of foam or the presence of culture media in a neck portion of the blood culture bottle. The image of the label is obtained from one or more images captured by a camera or scanner. The information on the label may be read automatically and therefore the blood culture bottles do not have to be processed manually, increasing throughput of the apparatus.

Abstract: The present invention describes an integrated incubator and image capture module that regulates the incubator atmosphere and obtains high-resolution digital images of sample specimens. The incubator has a cabinet type enclosure that enables the provision of a controlled environment to the contents of the incubator by having at least three ports on one face of the cabinet for the passage of sample containers. Additionally, an image capture module is located immediately adjacent to the incubator. In this regard, using at least three separate access/egress points for the sample containers streamlines operation of the system and enhances preservation of the incubator environment. Furthermore, locating the image capture module directly adjacent to the incubator reduces the amount of time a sample container is exposed to the external environment, thereby reducing the extent to which samples are exposed to potential contaminants and reducing the exchange of the lab and ambient atmospheres.

Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.

Abstract: A system and method for gripping, torqueing and releasing an element so as to cap and/or decap a container, such as those typically utilized to house specimens in laboratory environments. The system is driven by a single bi-directional motor linked to a coupler assembly via a rotating threaded shaft. The coupler assembly is configured to engage with an element, such as a cap or container, via mechanically-biased splines that are actuated without any complex linkages, or operative connection to the motor or other powered components. The system employs an ejector nut and an ejector, both of which are concentrically positioned about the threaded shaft. The ejector nut translates along the shaft as a function of the shaft's rotation, so as to permit the retraction of the ejector when an element is engaged in the coupler assembly or cause the ejector to extend into the coupler assembly to disengage the element.

Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.

Abstract: A system for capturing an image of a plated culture dish. The system includes an imaging device having a camera with a telecentric lens adapted to capture an image of the plated culture dish, a minor adapted to ensure that a label on the side of the plated culture dish is captured in an image of the plated culture dish that is captured by the imaging device. The system further includes at least one light system for illuminating the plated culture dish for image capture. The mirror is placed adjacent to the side of the plated culture dish on which the label is placed and at least a portion of the minor extends beneath a bottom portion of the plated culture dish at the side of the plated culture dish.

Abstract: A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.