Abstract: A method is described for identifying compounds in a complex mixture exhibiting a predetermined characteristic. The mixture is separated into fractions using at least two unique sets of separation parameters to produce at least two series of separation parameter dependent fractions. In one embodiment the mixtures are separated chromatographically using unique sets of separation parameters and the fractions are analyzed spectroscopically to provide data indicative of the component compounds and the fractions are analyzed in synchronously combined fractions, for the predetermined characteristic. The spectroscopic data for the fractions exhibiting the predetermined characteristic are compared to identify compound(s) common to the fractions exhibiting the characteristic.
Type:
Grant
Filed:
May 12, 2000
Date of Patent:
June 17, 2003
Assignee:
BDC Pharma LLC
Inventors:
Charles Pidgeon, Nadege M. Rooke, Jeffrey A. Ruell
Abstract: Methods are provided for determining a dissociation constant for each one of at least a portion of a set of compounds. The methods include contacting a solution of the set of compounds with a membrane mimetic surface exhibiting a non-specific compound-dependent affinity for at least the portion of the compounds. A parameter dependent on the non-specific affinity of the surface for each one of at least the portion of the set of compounds in the test solution is measured and used to calculate the dissociation constant.
Type:
Grant
Filed:
December 23, 1999
Date of Patent:
May 13, 2003
Assignee:
BDC Pharma LLC
Inventors:
Charles Pidgeon, Jianming Yin, Nadege Rooke, Ling Han Morgan