Abstract: The present invention fulfills the above stated needs, as well as others, by providing a automated container sealing apparatus that automatically removes seals from a backing and applies the seals to the container. The seals are pre-cut and have a size corresponding to the size of the container. In an exemplary embodiment, the present invention includes an apparatus for sealing containers, the containers defined by a generally planar sealing surface and one or more receptacles, wherein each of the one or more receptacles include an opening coplanar with and defining corresponding openings in the sealing surface.

Abstract: A device for the separation of the liquid portion of blood from the cellular components of blood comprises: (1) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood; (2) a substrate supporting the pad; and (3) means, attached to the pad, for facilitating the flow of the liquid portion of the blood: (i) through interstices around the trapped cellular components of the blood and (ii) from the pad of porous material. The separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad of porous material without significant hemolysis. The device can be incorporated into a device for the performance of specific binding assays such as immunoassays. The pad of porous material can contain an agglutinating agent such as a lectin or an anti-blood cell antibody, or a carbohydrate such as mannitol.

Abstract: A capping and sealing assembly for a sample-holding centrifuge container which features a self-sealing lid that forms a fluid-tight seal in an opening of the canister with a predetermined amount of force, with the force being dependent upon the centrifugal load to which the capping assembly is subjected. The lid includes a cover portion and a peripheral member surrounding the cover portion and extending transverse thereto. The lid is disposed within an open end of the container, with the peripheral member having a frusto-conical surface that faces the container's wall. A gasket is disposed about the frusto-conical surface, forming a fluid-tight and air-tight seal with the cylindrical wall of the canister. The gasket is received in a channel formed into the frusto-conical surface. A region of the channel includes a vent slot to facilitate removal of the container from the canister. A plurality of arcuate ribs extend across the cover portion, between opposed areas of the peripheral member.

Abstract: A system for a clinical lab that is capable of automatically processing, including sorting, of multiple specimen containers. The system comprises a central controller, a workstation, one or more analyzers, and an automated centrifuge. The workstation has automatic detectors for detecting the presence of a holder holding specimen containers. The workstation has a bar code reader for reading bar codes on the containers. The system has a transport subsystem, preferably a workstation robotic arm and an analyzer robotic arm for transporting the specimen containers, moving them to and from the workstation, to and from the analyzers, and to and from the centrifuge. The centrifuge is loaded with buckets containing specimen containers. The workstation can be provided with a balance system for balancing the weight of the buckets used. The workstation can also have a decapper for automatically removing caps from the specimen containers.

Abstract: The present invention relates to a method of immobilizing oligonucleotides and other biomolecules using a carboxylated polyvinylidene fluoride support membrane for the construction of geosensors and other array-based systems. The support membrane may be used in either covalent or non-covalent binding states.

Abstract: An automatic chemistry analyzer is provided having a high throughput and a high reliability. The analyzer uses nephelometric and turbimetric analyzers to analyze a wide variety of parameters within liquid samples typically generated in, for example, a large medical testing laboratory. The machine employs a unique probe and stirring rod assembly mounted at a slight angle with one another using rack and pinion assemblies so that the lower end of the probe and the lower end of the stirring rod are in very close proximity to one another. This feature allows the machine to be used in unusually small reaction cuvettes. The analysis machine also includes an onboard control sample so that the machine can be programmed to periodically calibrate its analyzing equipment during the course of normal operation.

Abstract: A centrifuge rotor locking apparatus includes a C-shaped annular member for a snap-fit engagement about a drive hub. The member comprises first and second contacting segments and a third non-contacting segment. Centrifugation causes the contacting segments to flex outwardly and come into contact with the interior wall of the drive hole of a mounted rotor. The unbalanced mass distribution of the annular member causes the member to tend to slide off the drive hub. This further separates the contacting segments, thus increasing the locking effect of the annular member/drive hub assembly to the rotor as the member is wedged in place between rotor and drive hub.

Abstract: A probe for heating a reagent and/or a test specimen from a storage temperature to an analysis temperature is provided herein. The probe is particularly useful with or as part of a clinical analyzer. The probe includes an electrically conductive, tubular inner structure and an electrically conductive, tubular outer structure which substantially encompasses the inner structure. An insulator is positioned between the inner structure and the outer structure which electrically separates the inner structure from the outer structure. Further, an interconnector electrically connects the inner structure to the outer structure. An electrical supply supplies current to the probe which flows through the inner structure and the outer structure to heat the reagent or the test specimen.

Abstract: A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component comprising a sample preparation means adapted to receive a sample to be assayed; and (2) a second opposable component comprising a chromatographic medium. The first and second opposable components can be brought into opposition so as to cause the sample preparation means to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes, as well as to permit bidirectional chromatography.

Abstract: Disclosed herein is a method for attaching pre-synthesized oligonucleotides to a polypropylene support medium. Most preferably, a polypropylene film is aminated by a plasma discharge in the presence of ammonia gas. An oligonucleotide having a terminal phosphate is activated in the presence of an imidazole and a carbodiimide to form a phosphorimidazolide. The activated oligonucleotide becomes immobilized by forming a phosphoramidate bond with the aminated polypropylene. The invention can be used to construct oligonucleotide arrays for hybridization assays.

Abstract: A device for the separation of the liquid portion of blood from the cellular components of blood comprises: (1) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood; (2) a substrate supporting the pad; and (3) means, attached to the pad, for facilitating the flow of the liquid portion of the blood: (i) through interstices around the trapped cellular components of the blood and (ii) from the pad of porous material. The separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad of porous material without significant hemolysis. The device can be incorporated into a device for the performance of specific binding assays such as immunoassays. The pad of porous material can contain an agglutinating agent such as a lectin or an anti-blood cell antibody, or a carbohydrate such as mannitol.

Abstract: An integrated apparatus for capillary electrophoresis which is automated using conveyors which carry vials containing electrolytes and samples, a capillary contained in a modular, portable, and interchangeable cartridge, and actuators for bringing the capillary into flow communication with the fluids in the vials. Electropotential is applied to the ends of the capillary to cause electrophoretic separation. The cartridge may include an opening to allow detection of fluid in the capillary using a detector provided in the apparatus.

Abstract: A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component comprising a sample preparation means adapted to receive a sample to be assayed; and (2) a second opposable component comprising a chromatographic medium. The first and second opposable components can be brought into opposition so as to cause the sample preparation means to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes, as well as to permit bi-directional chromatography.

Abstract: A stable self-forming density gradient is created by applying a centrifugal field to a solution that contains 27-33% Percoll (v/v) and 36-44% sugar (w/w). This density gradient is stable for several hours, allowing separation of low sedimentation particles, such as subcellular particles.

Abstract: An improved method for detecting hybridized biomolecules is provided which comprises the steps of, providing a plurality of probe biomolecules on a solid support surface; pre-treating the plurality of probe biomolecules with an effective amount of a pre-treatment solution to produce a signal having an increased intensity of at least two (2) times, as compared to an identical detection method that does not include the pre-treating step; applying a buffer solution to the probe biomolecules; hybridizing the probe biomolecules with a plurality of target biomolecules to form a hybridized complex; and, developing and detecting the signal intensity. The developing step of the method may further include the steps of, treating the hybridized complex with a conjugating solution, and treating the conjugated, hybridized complex with a detection reagent.

Abstract: A one piece, injection molded single piece, well container suitable for reagents for use in a clinical instrument such as a protein analyzer, normally molded from a high density polyethylene or other recyclable plastic. The container is one or more wells, uses standard caps, and is readily adapted to automatic filling equipment. The molded, single piece reagent cartridge is used in a clinical instrument, the reagent cartridge comprised of a plurality of storage wells located in a line in fixed relationship. The reagent cartridge has a guide at one end which is engageable with a means on the receiving means on an analyzer to restrain the cartridge from radial or lateral movement. Each of the wells has side walls and a closed bottom, and the side walls have an upper terminal position including an access opening.

Abstract: A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization to a reverse dot blot array comprising strings of such repeats complementary to those found in particular nucleic acid targets (e.g., analyte PCR product). An addressable library (i.e., an indexed set) of complementary repeats is synthesized on a suitable support. Preferably, the support comprises a low fluorescent background support, thereby facilitating the use of non-radioisotopic modes of detection (such as fluorescence or chemiluminescence); particularly suitable in this regard is an aminated polypropylene support or similar material. Preferred arrays permit screening of DNA and RNA samples for complete sets of particular types of nucleotide repeat sequences (e.g., all nucleotide doublet or triplet repeats).

Abstract: A rate nephelometer of the type useful in automated chemical analyzers is provided. The nephelometer includes a laser for generating a polarized laser beam having an S-wave component and a P-wave component. The beam is split by a beam splitter specially constructed so that a known proportion of one of the two polarized portions of the beam is directed to a reaction container. In the reaction container, a first polarized component of the laser beam is used in a nephelometric chemical analysis. The remainder of the laser beam passes through the beam splitter to a laser control light detector. Before the remainder of the laser beam reaches the laser control light detector, however, the polarized component which is not used in nephelometric chemical analysis is filtered out. The laser control detector uses the non-filtered portion of the laser beam to control the output of the laser.

Abstract: A hands-free gripping device which holds generally cylindrical containers, particularly labware, securely and prevents the container from turning while either opening or closing it. The gripping device features internal deformable ribbing, which secures the container by canting slightly when the container is turned thereby wedging the container in place. This action resists rotation in either direction, making a single device practical for both opening and closing. The device can be mounted on any flat surface and acts as a "third hand" to allow the user to use both hands or to use a single hand to grasp securely the closure being removed or secured from the container. The gripping device facilitates assembly or disassembly of labware devices needing application of torque.

Abstract: Methods of preparing a boronate-antigen complex for immunization of animals, a monoclonal antibody specific for the same, an immunoassay method for detection of the complex and a method of calculating the amount of a target glycated protein within the sample useful in the diagnostic monitoring of diabetes are disclosed. An immunoassay kit based on this reagent is also disclosed.