Abstract: Described are improved methods for treating solid tumors, such as malignant gliomas, with a dual approach utilizing a CAR-T immunotherapy and a viral oncolytic therapy.

Abstract: The present invention provides an apparatus and method for the amplification of a particular sequence(s) of DNA in a sample using polymerased chain reaction. The method of the present invention involves the injection of a reaction mixture into a stream of carrier fluid. The carrier fluid then passes through a plurality of temperature zones in which the polymerase chain reactions take place. The method of the present invention allows the sequential processing of a number of samples.

Abstract: A specific hybridoma cell line produces monoclonal antibodies which are effective in detecting carcinoembryonic antigens (CEA). The specific hydribome line and monoclonal antibodes are designated as T84.66-A3.1-H11. The monoclonal antibodies are preferably applied to tissues and fluids to detect the degree of binding of such monoclonal antibodies to such carcinoembryonic antigens.

Abstract: A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R(.sub.x)Si(NCS).sub.y where x=0-3 and y=1-4. R may be alkyl or aryl. The coupling reaction is preferably conducted at a temperature range of 50.degree.-90.degree. C. for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may also be utilized.

Abstract: A process for the detection of a particular polynucleotide sequence in a polynucleotide-containing test sample comprising forming an agarose gel having a depression therein, adding the test sample to said depression, putting agarose solution in the depression and permitting it to gel thereby to encase the test sample in agarose gel, denaturing the polynucleotide in the test sample within the agarose gel, under hybridization conditions contacting the test sample-containing gel with an oligonucleotide or polynucleotide probe having a sequence complementary with a sequence for which the sample is being tested, and determining the extent of hybridization.

Abstract: A method and apparatus for digitally analyzing continuous visual images, particularly with reference to the detection of mammalian cell mitotic events is disclosed. The visual images are analyzed by first extracting high frequency picture components, threshold comparison of such components and probing for annular objects indicative of putative mitotic cells. The detection of annulae is performed by an algorithm for recognizing rings of differential radii and compensating for other variations. Thereafter, spatial and temporal relationships between such objects is stored and compared to determine whether cell division occurred.