Abstract: Embodiments of the present disclosure may include an automated system for application of beehive treatment to a beehive, including a movable carriage, and a reservoir configured to hold the beehive treatment. Embodiments may also include a vision system disposed on the movable carriage and configured to detect an entrance of the beehive, an applicator system in fluid communication with the reservoir, the applicator system including an end effector configured to deliver the beehive treatment to the beehive through the entrance to the beehive. Embodiments may also include a controller in communication with the movable carriage, the vision system, and the applicator system.