Abstract: Provided in the present invention is a method for detecting copy numbers of survival of motor neuron genes SMN1 and/or SMN2 in a target genome, the method comprising: amplifying target regions in the SMN1 and/or SMN2 genes and an SMNP gene in a genome by using specific primer combinations, and then using an SMNP amplified product as a reference to determine the copy numbers of the SMN1 and/or SMN2 genes by means of comparing relative amounts of the amplified product. Further provided in the present invention is a reagent kit for detecting the copy numbers of SMN1 and/or SMN2.

Abstract: Disclosed are a detection system and a detection kit for the number of CGG unit repeats in the 5? untranslated region of genes. The detection system comprises three primers located upstream, downstream and at the boundary of the repeat of CGG repeats, and the number of CGG repeat units can be determined according to the results of the detected full-length product size and number of CGG product. In particular, since the primer located at the boundary of the repeating fragment has stronger binding ability to the corresponding template, the maximum CGG product can be determined more clearly, thereby the specific number of CGG repeats can be accurately determined, the number of repeats which is less than 60 repeats can be effectively and accurately determined, and it is possible to clearly determine whether there is a genotype with a larger number of repeats.