Abstract: The present invention relates to a method for direct measurement of high-density lipoprotein cholesterol. (HDL-C) or low-density lipoprotein cholesterol (LDL-C) based on a modified chemical precipitation method, wherein the improvements were made by optimizing the concentrations and proportions of potassium polyvinyl sulfate (PVSK) and polyethylene glycol methyl ether (PEGME) in such a way that the enzymatic reactions are completed before precipitation formations. The method in combination with certain surfactants showed good correlations with other direct methods used for HDL-C or LDL-C determinations.

Abstract: The present invention provides a method for reducing reagent usage, comprising the steps of providing a retainer, at least one group of loopholes being provided on the sidewalls of the retainer; providing a colorimetric glass, adapted to be inserted into the retainer, for receiving the first reagent to be observed through said loopholes; providing a cap, for capping the colorimetric glass and the retainer into a whole, after the colorimetric glass being inserted into the retainer. The present invention mainly uses a reagent tube with a retainer having a new structure, a colorimetric glass and a cap, storing the first reagent only in the colorimetric glass, and the volume of the colorimetric glass is far less than that of the current reagent tube, thus the usage of the reagents can be reduced effectively.

Abstract: The present invention provides a method for reducing reagent usage, comprising the steps of providing a retainer, at least one group of loopholes being provided on the sidewalls of said retainer; providing a colorimetric glass, adapted to be inserted into said retainer, for receiving the first reagent to be observed through said loopholes; providing a cap, for capping the colorimetric glass and the retainer into a whole, after the colorimetric glass being inserted into the retainer. The present invention mainly uses a reagent tube with a retainer having a new structure, a colorimetric glass and a cap, storing the first reagent only in the colorimetric glass, and the volume of the colorimetric glass is far less than that of the current reagent tube, thus the usage of the reagents can be reduced effectively.