Abstract: Provided herein are methods for increasing plant cell transformation efficiency. These methods include exposing the plant cells to a liquid medium containing a surfactant. Following exposure to the surfactant-containing medium, the cells can become more amenable to transformation and may be genetically transformed using methods known in the art. Exposure of the cells to the surfactant-containing medium prior to transformation can increase plant transformation efficiency when compared to transformation efficiency of cells not exposed to the surfactant-containing medium.

Abstract: Provided herein are compositions and methods for expressing a polynucleotide of interest in a plant or plant part. Compositions include nucleic acid molecules comprising a promoter sequence, and DNA constructs comprising the promoter molecule operably linked to one or more polynucleotides of interest. Plants and plant parts comprising the compositions or regenerated according to the methods are also provided.

Abstract: The present disclosure is directed to polynucleotides with a selection marker and a guide RNA module transcriptionally fused such that the selection marker and the genetic editing machinery are expressed concurrently. Accordingly, the selected cells are more likely to be genetically edited, such that the genetic editing efficiency is increased. The present disclosure also provides a method of transforming plant cells, a method of altering a target site in the genome of a plant cell, a method of increasing the fraction of genetically edited cells in a plant embryo following transformation using the polynucleotides.

Abstract: Provided herein are tomato plants and plant parts comprising altered expression, activity, or function of an SlAN2like transcription factor and/or an SlMYB-ATV transcription factor, and increased anthocyanin content. The tomato plants or plant parts provided herein can comprise one or more mutations in the Aft gene and the ATV gene. Also disclosed herein are compositions and methods of producing tomato plants and plant parts comprising increased anthocyanin content.

Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided.

Abstract: Provided herein are methods for increasing plant cell transformation efficiency. These methods include exposing the plant cells to a liquid medium containing a surfactant. Following exposure to the surfactant-containing medium, the cells can become more amenable to transformation and may be genetically transformed using methods known in the art. Exposure of the cells to the surfactant-containing medium prior to transformation can increase plant transformation efficiency when compared to transformation efficiency of cells not exposed to the surfactant-containing medium.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding DUF2996 domain-containing protein s, polypeptides encompassing DUF2996 domain-containing protein s, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: A method of harvesting plant product from a plant in a single pass using a combine harvester is disclosed. In the method, the plant has a protein content gradient that varies along a height of the plant. The method includes identifying, along a longitudinally-extending stalk of the plant, an upper protein gradient of the plant including high protein plant product and a lower protein gradient of the plant including lower protein plant product, wherein the high protein plant product from the upper protein gradient of the plant meets a threshold protein content that is higher than that of the lower protein plant product. The method also includes separately and substantially simultaneously harvesting the high protein plant product from the upper protein gradient and the lower protein plant product from the lower protein gradient in the single pass, and isolating the high protein plant product from the lower protein plant product.

Abstract: Provided herein are plants and plant parts containing mutation in LOX and/or FAD genes. Also disclosed are plants and plant parts comprising decreased LOX and/or FAD activity. Furthermore, provided herein are plants and plant parts, and products (e.g., protein compositions, oil) produced therefrom having reduced level of hexanal and/or hexanol, hexanol, linolenic acid, increased levels of oleic acid. Such plants, plant parts, and plant products can have improved flavor characteristics relative to the control WT plants. Plant oil having a high oleic acid content and a low linoleic/linolenic acid content is also provided. Also disclosed herein are methods and compositions of producing such plants and plant parts.

Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided.

Abstract: Compositions and methods for targeting pre-determined DNA sequences in bacterial cells are provided. The methods result in the targeted elimination of bacterial cells that comprise the pre-determined DNA sequence(s). Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. Methods to use these DNA constructs to selectively target and eliminate bacterial cells that harbor the targeted DNA sequence(s) are described herein.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ABC transporter proteins, polypeptides encompassing ABC transporter proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a targeted DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the targeted site(s). Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cpf1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined locations. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cpf1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding RING/U-Box superfamily proteins, polypeptides encompassing RING/U-Box superfamily proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: Compositions and methods for improving plant growth are provided herein. Disclosed herein are polypeptides encompassing m-type thioredoxin proteins, and polynucleotides encoding m-type thioredoxin proteins, wherein the thioredoxin protein-encoding sequence is expressed from mesophyll-preferred promoters. Also provided herein are expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding glutaredoxin proteins, polypeptides encompassing glutaredoxin proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: Compositions and methods for improving plant growth are provided herein. The maize RbcS7A promoter is provided which is a developmentally regulated promoter. Polynucleotides encoding ferredoxin-thioredoxin reductase proteins, polypeptides encompassing ferredoxin-thioredoxin reductase proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: Provided herein are methods for increasing plant cell transformation efficiency. These methods include exposing the plant cells to a liquid medium containing a surfactant. Following exposure to the surfactant-containing medium, the cells can become more amenable to transformation and may be genetically transformed using methods known in the art. Exposure of the cells to the surfactant-containing medium prior to transformation can increase plant transformation efficiency when compared to transformation efficiency of cells not exposed to the surfactant-containing medium.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing transcription factors (TFs) whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding photosystem I reaction center subunit N (PSAN) proteins, polypeptides encompassing PSAN proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.