Patents Assigned to Berry Genomics Co., Ltd.
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Patent number: 11959128Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.Type: GrantFiled: March 15, 2017Date of Patent: April 16, 2024Assignee: BERRY GENOMICS CO., LTD.Inventors: Xiaojie Zhang, Tao Cheng, Xiangbin Chen, Jianguang Zhang
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Patent number: 11535884Abstract: The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.Type: GrantFiled: February 7, 2018Date of Patent: December 27, 2022Assignee: BERRY GENOMICS CO., LTD.Inventors: Ziwei Qu, Tao Yu, Fangming Wang, Nannan Zhang, Jianguang Zhang
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Publication number: 20200263235Abstract: The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.Type: ApplicationFiled: February 7, 2020Publication date: August 20, 2020Applicant: BERRY GENOMICS CO., LTDInventors: Ziwei QU, Tao YU, Fangming WANG, Nannan ZHANG, Jianguang ZHANG
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Patent number: 10648037Abstract: The present invention discloses a method for non-invasively detecting EGFR gene mutations in subjects, comprising the following steps: designing primers according to EGFR gene exons; extracting plasma DNAs in subjects; connecting the extracted plasma DNAs with tagging linkers; PCR pre-amplifying the tagging linkers connected plasma DNAs; cyclising the pre-amplified DNAs to obtain cyclised DNAs; PCR amplifying the cyclised DNAs using the designed primers; and high throughput sequencing the PCR amplified product and analyzing the EGFR gene mutations. The present invention also discloses a corresponding kit.Type: GrantFiled: December 16, 2014Date of Patent: May 12, 2020Assignee: Berry Genomics Co., Ltd.Inventors: Lina Wang, Lanlan Ru, Tiancheng Li, Yaxi Zhang, Jianguang Zhang
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Publication number: 20200131507Abstract: Provided herein is a method of constructing a high throughput sequencing library for use in detecting chromosome copy number variation comprising mainly the following steps: (1) subjecting DNAs to be tested to random fragmentation by a double-strand DNA fragmentation enzyme; (2) end-filling and adding poly-adenine at the 3?end of the fragmented DNAs; (3) connecting the end-filled DNAs having a 3?end poly-adenine with sequencing linkers to obtain connected products; (4) purifying the connected products to obtain the sequencing library; wherein steps (1)-(3) are performed in a single reaction tube. Also provided is a kit for constructing a sequencing library for use in detecting chromosome copy number variation.Type: ApplicationFiled: December 11, 2017Publication date: April 30, 2020Applicant: Berry Genomics Co., Ltd.Inventors: Ling Shang, Meng Lv, Xiangbin Chen, Tingting Wang, Jianguang Zhang
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Publication number: 20200080138Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.Type: ApplicationFiled: March 15, 2017Publication date: March 12, 2020Applicant: BERRY GENOMICS CO., LTDInventors: Xiaojie ZHANG, Tao CHENG, Xiangbin CHEN, Jianguang ZHANG
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Patent number: 10227587Abstract: The disclosure relates to a method for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5?-terminus phosphorylation for the plasma DNA.Type: GrantFiled: November 18, 2016Date of Patent: March 12, 2019Assignee: Berry Genomics Co., Ltd.Inventors: Jianguang Zhang, Yang Gao, Yanbin Shi, Di Chen, Feng Tian
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Publication number: 20190062731Abstract: The disclosure relates a method for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5?-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5?-terminus phosphorylation for the plasma DNA.Type: ApplicationFiled: November 18, 2016Publication date: February 28, 2019Applicant: BERRY GENOMICS CO., LTD.Inventors: Jianguang Zhang, Yang Gao, Yanbin Shi, Di Chen, Feng Tian
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Patent number: 9885080Abstract: The invention relates to a kit, a device and a method for detecting the copy number of fetal chromosomes and tumor cell chromosomes.Type: GrantFiled: May 31, 2011Date of Patent: February 6, 2018Assignee: BERRY GENOMICS CO., LTD.Inventors: Yang Gao, Daixing Zhou, Jianguang Zhang, Feng Tian
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Patent number: 9540687Abstract: The disclosure claims a cleaved Deoxyribonucleic acid (DNA) detection method, a DNA fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the DNA fragment; cyclizing the DNA fragment to obtain acyclized DNA; implementing Polymerase Chain Reaction (PCR) amplification for the cyclized DNA by using the primers; and detecting the PCR amplification product. In the disclosure, by cyclizing the DNA fragment, the amplification can be implemented even if only one PCR primer can match with a template, thus, the adaption range and effective template amount of the primer amplification can be greatly increased, and the detection sensitivity of the DNA fragment can be greatly improved.Type: GrantFiled: July 11, 2012Date of Patent: January 10, 2017Assignee: BERRY GENOMICS CO., LTD.Inventors: Jianguang Zhang, Zhongchun Zhou, Daixing Zhou, Genming Xu, Yang Gao, Feng Tian
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Publication number: 20150307942Abstract: The present invention is directed to a method, kit and primers for detecting fetal deafness pathogenic gene mutations. The method of the invention comprises: (a) designing primers according to the pre-determined mutation loci of deafness pathogenic genes; (b) extracting plasma DNAs in a pregnant woman; (c) connecting the extracted plasma DNAs with pre-amplification linkers to obtain connected products; (d) PCR pre-amplifying the connected product to obtain pre-amplified products; (e) cyclizing the pre-amplified products to obtain cyclised DNAs; (f) PCR amplifying the cyclised DNAs using the designed primers to obtain amplified products; and (g) high throughput sequencing the amplified products and analyzing the mutations of the fetal deafness pathogenic genes. The invention can effectively determine whether the pre-determined loci on deafness pathogenic genes have been mutated as well as the mutation type.Type: ApplicationFiled: April 22, 2015Publication date: October 29, 2015Applicant: BERRY GENOMICS CO., LTD.Inventors: Yiqian Liu, Xianchao Guo, Yong Fu, Yugang Hu, Tiancheng Li, Jianguang Zhang
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Publication number: 20140234850Abstract: The disclosure claims a cleaved Deoxyribonucleic acid (DNA) detection method, a DNA fragment detection kit and use thereof. Wherein, the method includes the steps of: designing primers according to a test site or a test region of the DNA fragment; cyclizing the DNA fragment to obtain acyclized DNA; implementing Polymerase Chain Reaction (PCR) amplification for the cyclized DNA by using the primers; and detecting the PCR amplification product. In the disclosure, by cyclizing the DNA fragment, the amplification can be implemented even if only one PCR primer can match with a template, thus, the adaption range and effective template amount of the primer amplification can be greatly increased, and the detection sensitivity of the DNA fragment can be greatly improved.Type: ApplicationFiled: July 11, 2012Publication date: August 21, 2014Applicant: Berry Genomics Co., LtdInventors: Jianguang Zhang, Zhongchun Zhou, Daixing Zhou, Genming Xu, Yang Gao, Feng Tian
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Publication number: 20140228256Abstract: The disclosure relates a method and a kit for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method provided by the disclosure includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5?-terminus phosphorylation for the plasma DNA.Type: ApplicationFiled: January 10, 2012Publication date: August 14, 2014Applicant: BERRY GENOMICS CO., LTD.Inventors: Jianguang Zhang, Yang Gao, Yanbin Shi, Di Chen, Feng Tian
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Publication number: 20130130921Abstract: The invention relates to a kit, a device and a method for detecting the copy number of fetal chromosomes and tumor cell chromosomes.Type: ApplicationFiled: May 31, 2011Publication date: May 23, 2013Applicant: Berry Genomics Co., Ltd.Inventors: Yang Gao, Daixing Zhou, Jianguang Zhang, Feng Tian