Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.
Abstract: Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.
November 10, 2015
October 4, 2018
BGI Shenzhen Co., Ltd.
Handong LI, Y. Tom TANG, Jing YU, Hui JIANG, Wenwei ZHANG, Guangyi FAN, He ZHANG, Kailong MA, Chunyu GENG
Abstract: The present invention provides a high-throughput sequencing method for methylated DNA, and use thereof. Particularly, the present invention provides a high-throughput sequencing method for methylated DNA, which combines methylated DNA immunoprecipitation, removal of repetitive sequences, and bisulfite treatment. The site of sequencing library will be decreased, and the cost will be reduced by using the method disclosed in the present invention.
August 11, 2010
Date of Patent:
December 13, 2016
Institute of Psychology, Chinese Academy of Sciences, BGI Shenzhen Co., Ltd.
Yan Wang, Mingzhi Ye, Xu Han, Xiuqing Zhang, Zhongsheng Sun
Abstract: A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
December 29, 2011
January 16, 2014
BGI-Shenzhen Co., Ltd.
Xuyang Yin, Li Bao, Xun Xu, Hanjie Wu, Xiaoyu Liu, Xiuqing Zhang, Huanming Yang