Patents Assigned to BIA Separations d.o.o.
  • Patent number: 12227738
    Abstract: A method of single-stranded RNA purification comprising the steps applying a sample containing single-stranded RNA to a solid phase bearing dominantly or exclusively primary amino groups on its surface, at a pH value sufficient to bind at least predominantly the single-stranded RNA, eluting the single-stranded RNA from the surface of the solid phase by exposing the surface of the solid phase to increasing pH.
    Type: Grant
    Filed: April 16, 2021
    Date of Patent: February 18, 2025
    Assignee: Sartorius BIA Separations d.o.o.
    Inventors: Špela Peršič Debenjak, Urh Černigoj, Darko Dolenc, Peter S. Gagnon
  • Patent number: 10597640
    Abstract: The present invention discloses a novel composition of the elution mobile phase for virus purification by immunoaffinity chromatography which is consisting of one or more amino acids: L-serine, L-asparagine, or L-glutamine, or their salts with pharmaceutically acceptable acids; one or more auxiliary ingredients: L-arginine, glycine or imidazole, or their salts with pharmaceutically acceptable acids; one or more pharmaceutically acceptable pH adjusting agents for correcting the pH value of the mobile phase from pH=6.0-8.0; and purified water, up to 100% w/w of the mobile phase composition. The invention provides the use of immunoaffinity chromatography as a key step in the production of viral vaccines and/or viral vectors, in separation of infectious from non-infectious viral particles, and for enrichment of the viral suspension in infectious viral particles.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: March 24, 2020
    Assignees: Sveuciliste U Zagrebu, Bia Separations d.o.o.
    Inventors: Marija Brgles, Beata Halassy, Dubravko Forcic
  • Patent number: 6736973
    Abstract: A porous self-supporting structure comprising at least two porous components A and B and the porous component B embraces the porous component A, wherein (i) surfaces of pores of the at least two porous components A and B are provided with chemical moieties for interaction with substances passing the pores and (ii) the pores of the porous components comprise a uniform multimodal pore size distribution through the entire polymeric structure.
    Type: Grant
    Filed: July 26, 2000
    Date of Patent: May 18, 2004
    Assignee: BIA Separations d.o.o.
    Inventors: Ales Podgornik, Milos Barut, Ales Strancar, Djuro Josic
  • Publication number: 20040002081
    Abstract: The invention relates to a process for isolating and purifying a polynucleotide on a manufacturing scale which uses a chromatographic separation process comprising a combination of two different chromatographic steps selected from hydrophobic interaction chromatography, polar interaction chromatography and anion exchange chromatography. In at least one of the two steps the chromatographic support is a porous monolithic bed. The invention also relates to a chromatographic device and its use for isolating and purifying a polynucleotide of interest, in particular plasmid DNA, on manufacturing scale.
    Type: Application
    Filed: December 18, 2002
    Publication date: January 1, 2004
    Applicant: Boehringer Ingelheim International GmbH and BIA Separations d.o.o.
    Inventors: Jochen Urthaler, Roman Necina, Janez Jancar, Ales Podgornik, Milos Barut, Ales Strancar, Mojca Merhar
  • Patent number: 6664305
    Abstract: A process of manufacturing of a chromatography material comprising the steps of (i) reacting a polymerizable at least bifunctional monomer A with a ligand also having a functional group which binds covalently with one of the functional groups of said polymerizable bifunctional monomer A, (ii) to form a compound B comprising at least one polymerizable functional moiety (iii) polymerizing the compound B essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting or (iv) reacting the ligand and a spacer via a covalent bond which is cleavable to form a ligand-spacer conjugate and binding the ligand-spacer conjugate to the surface of a chromatographic support or reacting the ligand-spacer conjugate via a covalent bond to the at least bi-functional monomer A and polymerizing it essentially alone or with the polymerizable monomers in presence of porogenes to obtain a block of porous polymerizate which is self-supporting.
    Type: Grant
    Filed: October 28, 2002
    Date of Patent: December 16, 2003
    Assignee: BIA Separations d.o.o.
    Inventors: Alois Jungbauer, Rainer Hahn, Ales Podgornik, Milos Barut, Ales Strancar
  • Patent number: 6319401
    Abstract: A device for the rapid separation and/or conversion of substrates is disclosed herein. The device include a porous tube (2) disposed inside a tubular sample distributor (3). The tubular sample distributor (3) includes channel structures on its inner surface and is disposed inside a tubular body (4). A tubular sample collector (1) having channel structures on its outer surface is disposed inside the porous tube (2). A circular sample distributor (7) is provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample distributor (3). A circular sample collector (8) is also provided having a circumferential portion and radially extending grooves in conjunction with the channel structures of the tubular sample collector (1). The hollow body (4) is arranged between securing means (5) that provide a sample inlet and outlet in communication with the circular sample distributor (7) and circular sample collector (8), respectively.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: November 20, 2001
    Assignee: BIA Separations d.o.o.
    Inventors: Djuro Josic, Primoz Koselj, Ales Podgronik, Ales Strancar