Abstract: The present invention relates to a fusion protein containing an erythropoietin polypeptide fused to the Fc region of mammal-derived IgG (hereinafter to be also abbreviated as EPO-Fc fusion protein). The fusion protein in which erythropoietin polypeptide has the amino acid sequence shown in SEQ ID NO: 9 or 10, and the Fc region has the amino acid sequence shown in SEQ ID NO: 3 or 4 is more superior in the property and activity and can be obtained at a high yield.