Abstract: Fetal bovine serum (FBS) is considered a major supplement in culturing cells. Due to ethical issues, high costs, and batch-to-batch variations, designing an alternative to the FBS in cell culturing is important. A biological supplement including of microbiota-derived postbiotics (MD-PBs) obtained from a fermentation medium of beneficial microorganisms isolated from natural microbiota sources was formulated, processed, combined, and used as an alternative to the FBS in stimulating cell proliferation and growth. According to results obtained from different cell lines and primary cells, the MD-PBs and exopolysaccharides (EPSs) alone and/or Bovine Serum Albumin (BSA) and Sericin stimulated cell growth better than the FBS and, thus the MD-PBs and the EPSs alone and/or the BSA and Sericin are used as a possible alternative to the FBS in cell culture experiments and cultured meat technology.