Patents Assigned to Bigtec Private Limited
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Patent number: 10525461Abstract: The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.Type: GrantFiled: August 30, 2016Date of Patent: January 7, 2020Assignee: Bigtec Private LimitedInventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
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Patent number: 10227633Abstract: The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.Type: GrantFiled: October 7, 2016Date of Patent: March 12, 2019Assignee: BIGTEC PRIVATE LIMITEDInventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
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Patent number: 9404151Abstract: The present disclosure provides a non-contact real time micro Polymerase Chain Reaction (PCR) system comprising; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising an associated LED driver and a photo detector amplifier placed above the chip to detect fluorescence; an induction heater mounted around the chip and inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein the infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.Type: GrantFiled: April 28, 2011Date of Patent: August 2, 2016Assignee: BIGTEC PRIVATE LIMITEDInventors: Swetha Chandra, Sudip Mondal, Venkataraman Venkatakrishnan, Sathyadeep Viswanathan, Renjith Mahiladevi Radhakrishnan, Raviprakash Jayaraman, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Patent number: 9370774Abstract: Instant invention is about a hand held micro PCR device comprising a LTCC micro PCR chip comprising a heater, a reaction chamber to load a sample. It also comprises a heater control to regulate the heater on basis of input received from a temperature sensor. It further has an optical system having an optical fiber to detect a fluorescence signal from the sample, and at least one communication interface to interact with other device(s).Type: GrantFiled: October 13, 2008Date of Patent: June 21, 2016Assignee: Bigtec Private LimitedInventors: Kishore Krishna Kumar, Raviprakash Jayaraman, Sankaranand Kaipa Narasimha, Renjith Mahiladevi Radhakrishnan, Sathyadeep Viswanathan, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao, Manjula Jagannath, Shilpa Chennakrishnaiah, Sudip Mondal, Venkatakrishnan Venkataraman
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Patent number: 9309281Abstract: In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.Type: GrantFiled: August 22, 2013Date of Patent: April 12, 2016Assignee: Bigtec Private LimitedInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Patent number: 9238674Abstract: In one aspect, methods relate to the field of recombinant DNA therapeutics. Methods may involve bio-informatics design, synthesis of artificial genes for a human insulin precursor having a leader peptide coding sequence, cloning artificial genes into an expression vector, and expression in an organism such as one selected from the genus Pichia. In another aspect, methods may include downstream processing for obtaining protein precursor molecules and subsequent conversion of protein precursor molecules to functional proteins.Type: GrantFiled: August 22, 2013Date of Patent: January 19, 2016Assignee: Bigtec Private LimitedInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Patent number: 9228144Abstract: The present invention relates to a biofuel composition comprising normal chain alcohol(s), natural oils and diesel optionally along with fuel additive(s); a process of preparation and a method of fueling said biofuel composition.Type: GrantFiled: August 10, 2009Date of Patent: January 5, 2016Assignee: BIGTEC PRIVATE LIMITEDInventors: Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao, Phani Kumar Pullela, Gopalakrishna Mangalore Kini
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Patent number: 9044754Abstract: Instant invention is about a micro chip comprising plurality of layers of LTCC wherein a reaction chamber is formed in plurality of top layers to load samples. A heater embedded in at least one of the layers below the reaction chamber and a temperature sensor is embedded in at least one of the layers between the heater and the reaction chamber for analyzing the sample. The temperature sensor can be placed outside the chip to measure the chip temperature.Type: GrantFiled: October 13, 2008Date of Patent: June 2, 2015Assignee: Bigtec Private LimitedInventors: Kishore Krishna Kumar, Raviprakash Jayaraman, Sankaranand Kaipa Narasimha, Renjith Mahiladevi Radhakrishnan, Sathyadeep Viswanathan, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao, Manjula Jagannath, Shilpa Chennakrishnaiah
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Patent number: 8986976Abstract: The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.Type: GrantFiled: January 6, 2011Date of Patent: March 24, 2015Assignee: Bigtec Private LimitedInventors: Phani Kumar Pullela, Mulakkapurath Narayanan Manoj, Santhosh Kumar Gandhavalla, Mitchell Preetham Pinto, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20140057318Abstract: The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.Type: ApplicationFiled: August 22, 2013Publication date: February 27, 2014Applicant: BIGTEC PRIVATE LIMITEDInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20140051842Abstract: The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.Type: ApplicationFiled: August 22, 2013Publication date: February 20, 2014Applicant: BIGTEC PRIVATE LIMITEDInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Patent number: 8575322Abstract: The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.Type: GrantFiled: March 12, 2010Date of Patent: November 5, 2013Assignee: Bigtec Private LimitedInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20130203150Abstract: The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.Type: ApplicationFiled: January 6, 2011Publication date: August 8, 2013Applicant: BIGTEC PRIVATE LIMITEDInventors: Phani Kumar Pullela, Mulakkapurath Narayanan Manoj, Santhosh Kumar Gandhavalla, Mitchell Preetham Pinto, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20130130235Abstract: The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof.Type: ApplicationFiled: July 15, 2011Publication date: May 23, 2013Applicant: BIGTEC PRIVATE LIMITEDInventors: Manjula Jagannath, Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20130101983Abstract: The present disclosure provides a non contact real time micro Polymerase Chain Reaction [PCR] system comprises; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising associated LED driver and photo detector amplifier placed above the chip to detect the fluorescence; an induction heater mounted around the chip and is inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein said infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.Type: ApplicationFiled: April 28, 2011Publication date: April 25, 2013Applicant: BIGTEC PRIVATE LIMITEDInventors: Swetha Chandra, Sudip Mondal, Venkataraman Venkatakrishnan, Sathyadeep Viswanathan, Renjith Mahiladevi Radhakrishnan, Raviprakash Jayaraman, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Patent number: 8282692Abstract: The present invention relates to a biofuel composition comprising distilled technical cashew nut shell liquid (DT-CNSL) and atleast one petroleum product optionally alongwith plant oils and fuel additive(s). It also relates to a process for preparation of biofuel composition and a method of fueling biofuel composition.Type: GrantFiled: June 26, 2008Date of Patent: October 9, 2012Assignee: Bigtec Private LimitedInventors: Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao, Phanikumar Pullela, Gopalkrishna Mangalore Kini
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Publication number: 20120077966Abstract: The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.Type: ApplicationFiled: March 12, 2010Publication date: March 29, 2012Applicant: BIGTEC PRIVATE LIMITEDInventors: Mulakkapurath Narayanan Manoj, Venkata Ramachandra Rao Vasamsetty, Madhuri Baliga, Kirubakaran Naveen Kumar, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20120045761Abstract: The present disclosure gives a detailed description of methods for determining the presence of Chikungunya viral nucleic acids in blood/serum/plasma samples by employing “Oligonucleotide” probes. The designed “Oligonucleotide” probes can be used for qualitative or quantitative detection of Chikungunya virus in an infected sample by employing Real time PCR.Type: ApplicationFiled: February 23, 2010Publication date: February 23, 2012Applicant: BIGTEC PRIVATE LIMITEDInventors: Manjula Jagannath, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20110306037Abstract: The present disclosure provides a method for the detection and quantification of Hepatitis B Virus. It discloses oligonucleotide probes set forth in SEQ ID Nos. 1 and 2 for detection of Hepatitis B Virus along with respective primers [sense and antisense] set forth in SEQ ID Nos. 3, 4, 5 and 6. It also provides a PCR reaction mixture for detection of Hepatitis B Virus and a kit for detection of HBV comprising said mixture along with an instruction package.Type: ApplicationFiled: January 27, 2010Publication date: December 15, 2011Applicant: BIGTEC PRIVATE LIMITEDInventors: Manjula Jaggannath, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
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Publication number: 20110306046Abstract: The present disclosure gives description of a method used for the detection and quantification of malarial infection caused either by Plasmodium falciparum or Plasmodium vivax using nucleic acids isolated from blood samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR.Type: ApplicationFiled: January 27, 2010Publication date: December 15, 2011Applicant: BigTec Private LimitedInventors: Manjula Jagannath, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao, Mulakkapurath Narayanan Manoj, Shamamandri Markandaiah Shashirekha