Abstract: The present invention relates to an automatic DNA isolation apparatus which comprises liquid containers; numerous liquid flow passages; control valves; numerous syringes; numerous injection needles; transportation means; vacuum block; plate rack (31); numerous multi-well plate blocks; and process control unit which regulates the operation of said control valves for selection and transfer of said various liquids, the operation of said syringe for quantitative intake and discharge of said liquids, the transfer of said injection needle connected with said syringes, the sequential transportation of said multi-well plates arranged in said multi-well plate rack, the sequential operation of shaking block and said vacuum block.