Abstract: Embodiments of this invention include image-based systems and methods for detection of one or more biological molecules. A matrix has identifiable analyte-specific capture particle(s) suspended therein, not all being in the same detection plane. Analyte-specific binding moieties (e.g., antibodies) capture analytes. Analyte-specific detector molecules with conjugated detection moieties are then attached to the analyte-specific binding moiety forming particle complexes. A computer assisted, image-based detection system captures images of the particle complexes. Using different sets of analyte-specific detector molecules, each set having a characteristic identifiable feature; it is now possible to implement rapid multiplex assays of cytokines. These image-based systems can be used to aid in diagnosis of disease, evaluation of therapy for disease, or laboratory investigation.

Abstract: Embodiments of this invention include image-based systems and methods for detection of one or more analytes. A surface has identifiable analyte-specific capture particle(s) immobilised thereto at any point of an assay, to which different analytes attach due to the affinity of analyte-specific capture molecule(s) linked to the surface of the capture particle(s) for the analyte. Analyte-specific detector molecules with conjugated detection moieties are then attached to the analyte, and a computer assisted, image-based detection system captures images of the capture particles with or without attached analytes and detector molecules. By using different subsets of analyte-specific capture molecules, each subset having a characteristic identifiable feature; it is now possible to perform capture particle-based, rapid multiplex assays of biological and non-biological analytes without flow.