Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Type:
Grant
Filed:
May 22, 2001
Date of Patent:
March 16, 2004
Assignee:
Bio101, Inc.
Inventors:
James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Type:
Grant
Filed:
September 25, 1997
Date of Patent:
May 22, 2001
Assignee:
Bio101, Inc.
Inventors:
James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana