Patents Assigned to Bio harm Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka mbH
  • OPTIMIZED PURIFICATION PROCESS OF RECOMBINANT GROWTH FACTOR PROTEIN
    Publication number: 20110160441
    Abstract: A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.
    Type: Application
    Filed: June 20, 2008
    Publication date: June 30, 2011
    Applicant: Bio harm Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka mbH
    Inventors: Ute Ehringer, Eva Kohlstrung