Patents Assigned to Bio-Pixels Ltd.
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Patent number: 6828142Abstract: Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complementary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission.Type: GrantFiled: July 13, 2001Date of Patent: December 7, 2004Assignee: Bio-Pixel Ltd.Inventors: Emillo Barbera-Guillem, M. Bud Nelson, Stephanie L. Castro
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Patent number: 6333110Abstract: Provided is a method of fluorescence imaging of living tissue using functionalized nanocrystals. The method comprises contacting an effective amount of functionalized nanocrystals with the living tissue; exposing the tissue to a spectrum of light suitable for exciting functionalized nanocrystals, present in fluorescently labeled tissue, to emit a emission spectrum comprising a fluorescence peak; and detecting any fluorescence peak emitted by the tissue exposed to the excitation spectrum of light, and obtaining a fluorescence image of the tissue. Also provided is a composition comprising a functionalized nanocrystal which is bound to a substrate in a living tissue.Type: GrantFiled: September 13, 1999Date of Patent: December 25, 2001Assignee: Bio-Pixels Ltd.Inventor: Emilio Barbera-Guillem
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Patent number: 6319607Abstract: Provided are methods for purifying functionalized fluorescent nanocrystals having affinity ligand operably bound thereto by using a solid support matrix in a reactor through which solutions are circulated, and by using an immobilized solid phase formed by operably binding functionalized fluorescent nanocrystals to the solid support matrix. Also provided are purified functionalized fluorescent nanocrystals having affinity ligand operably bound thereto which are purified using the methods according to the present invention to be substantially free of free affinity ligand.Type: GrantFiled: June 8, 2000Date of Patent: November 20, 2001Assignee: Bio-Pixels Ltd.Inventors: Emilio Barbera-Guillem, Stephanie L. Castro
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Patent number: 6309701Abstract: Provided are a fluorescent microsphere comprised of a plurality of fluorescent nanocrystals operably bound to a polymeric microsphere, and a method of producing the fluorescent microspheres which comprises contacting the polymeric microsphere with a plurality of fluorescent nanocrystals under suitable conditions in which the fluorescent naocrystals become operably bound to the polymeric microsphere. Also provided is a method of using the fluorescent microspheres capable of determining the presence or absence of a predetermined number of analytes in a sample by contacting the sample with the fluorescent microspheres, and detecting the fluorescence signal pattern of excited fluorescent microspheres bound to one or more analytes of the predetermined number of analytes, if present in the sample.Type: GrantFiled: May 24, 2000Date of Patent: October 30, 2001Assignee: Bio-Pixels Ltd.Inventor: Emilio Barbera-Guillem
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Patent number: 6261779Abstract: Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complemetary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission.Type: GrantFiled: November 9, 1999Date of Patent: July 17, 2001Assignee: Bio-Pixels Ltd.Inventors: Emilio Barbera-Guillem, M. Bud Nelson, Stephanie L. Castro
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Functionalized nanocrystals and their use in labeling for strand synthesis or sequence determination
Patent number: 6221602Abstract: Provided are compositions comprising functionalized nanocrystal-labeled nucleobases that are produced by operably linking a nucleobase to a functionalized nanocrystal via reactive functionalities. Also provided are kits comprising a plurality of species of functionalized nanocrystal-labeled nucleobases. The functionalized nanocrystal-labeled nucleobases may be added to a strand synthesis reaction under suitable conditions to be incorporated into one or more strands synthesized in the reaction.Type: GrantFiled: November 9, 1999Date of Patent: April 24, 2001Assignee: Bio-Pixels Ltd.Inventors: Emilio Barbera-Guillem, M. Bud Nelson, Stephanie L. Castro -
Patent number: 6203758Abstract: A micro-circuit for performing analyses of multimolecular interactions and for performing molecular syntheses, comprising: (a) a support; (b) at least one micro-electrode attached to the support, the micro-electrode being selectively electronically activated and the micro-electrode having a protective layer which is removable; (c) a binding entity for attachment to the at least one micro-electrode, the binding entity being capable of attachment to at least one micro-electrode when the protective layer has been removed; and (d) a power source being operatively connected to at least one micro-electrode for electronically activating at least one micro-electrode. The micro-circuit of the present invention also includes embodiments featuring a micro-circuit reader for detecting the interaction of the binding entity to a complementary probe, as well as methods for making and using the micro-circuit of the present invention.Type: GrantFiled: November 10, 1997Date of Patent: March 20, 2001Assignee: Bio-Pixel Ltd.Inventors: Robert Marks, Jean-Paul Lellouche
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Patent number: 6194213Abstract: Provided is a method of fluorescence detection of lipid membranes using lipophilic, functionalized nanocrystals; and lipid membranes labeled by the lipophilic, functionalized nanocrystals. The method comprises contacting an effective amount of lipophilic, functionalized nanocrystals with the lipid membranes; exposing the labeled lipid membranes to an excitation light suitable for exciting the functionalized nanocrystals present in the labeled lipid membranes to emit a fluorescence emission. Also provided is a composition comprising a functionalized nanocrystal which is bound to a substrate in a living tissue.Type: GrantFiled: December 10, 1999Date of Patent: February 27, 2001Assignee: Bio-Pixels Ltd.Inventor: Emilio Barbera-Guillem