Patents Assigned to Bio-Rad Laboratories
  • Patent number: 11939573
    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
    Type: Grant
    Filed: July 22, 2020
    Date of Patent: March 26, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Serge Saxonov
  • Publication number: 20240082847
    Abstract: Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.
    Type: Application
    Filed: November 22, 2023
    Publication date: March 14, 2024
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Darren R. LINK
  • Patent number: 11926871
    Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.
    Type: Grant
    Filed: March 29, 2021
    Date of Patent: March 12, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Lucas Frenz, Jeremy Agresti
  • Patent number: 11917737
    Abstract: A circuit for sharing current between parallel LEDs or parallel strings of LEDs, and a method of use of the same, are disclosed herein. The circuit for sharing current between parallel LED pathways can include a first LED pathway, a first transistor coupled to the first set of LEDs and that can control a first current through the first set of LEDs, and a first measurement node having a first sensed voltage. The circuit can include a second LED pathway, a second transistor coupled to the second set of LEDs and that can control a second current through the second set of LEDs, and a second measurement node having a second sensed voltage. The circuit includes a first differential amplifier and a second differential amplifier, each of which can compare sensed voltage and can apply a voltage to a gate of one of the first and second transistors.
    Type: Grant
    Filed: July 29, 2022
    Date of Patent: February 27, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Edward George Marquette
  • Patent number: 11913954
    Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.
    Type: Grant
    Filed: February 8, 2021
    Date of Patent: February 27, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ravi Kaul, Weiming Zheng, Roger Walker
  • Patent number: 11905551
    Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.
    Type: Grant
    Filed: October 22, 2021
    Date of Patent: February 20, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti, George Karlin-Neumann
  • Patent number: 11905335
    Abstract: Methods and kits for purifying protein-nanoparticle conjugates are provided. In some embodiments, a multimodal medium having a size exclusion mode and a capture mode is used to purify the protein-nanoparticle conjugates.
    Type: Grant
    Filed: February 2, 2021
    Date of Patent: February 20, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jiali Liao, Tom Berkelman
  • Patent number: 11901041
    Abstract: In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: February 13, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Michael Samuels, Jeffrey Olson, Darren R. Link
  • Patent number: 11896969
    Abstract: Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: February 13, 2024
    Assignee: BIO-RAD LABORATORIES, INC.
    Inventors: Christopher Belisle, Xuemei He, Hong Chen, Yueping Xu, Jiali Liao
  • Patent number: 11898201
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Grant
    Filed: January 13, 2021
    Date of Patent: February 13, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Nicholas Heredia, Dianna Maar
  • Patent number: 11898193
    Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.
    Type: Grant
    Filed: September 10, 2019
    Date of Patent: February 13, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Benjamin J. Miller, Qun Zhong, Darren Roy Link
  • Publication number: 20240044926
    Abstract: A clinical diagnostic analyzer for performing an automated crossover study on quality control (QC) material includes a processor, memory, measurement hardware, and an input panel/display. The analyzer prompts a user to load a QC specimen, and to instigate testing and analysis to determine a mean and a standard deviation for the new material. Associated methods for using one or more clinical diagnostic analyzers to calculate a new mean and standard deviation for a new QC material, reduce error in the calculated mean value, and to reduce the total number of days to complete a crossover study are also disclosed.
    Type: Application
    Filed: December 21, 2020
    Publication date: February 8, 2024
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: John Yundt-Pacheco, Curtis Parvin, Nico VandePoele
  • Patent number: 11892205
    Abstract: A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.
    Type: Grant
    Filed: November 18, 2022
    Date of Patent: February 6, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Amir Sadri, Nenad Kircanski, Thanh-Vi Tran, Carl Marlowe, Brian David Wilson
  • Patent number: 11878302
    Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
    Type: Grant
    Filed: September 2, 2021
    Date of Patent: January 23, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: George Carman, Thomas H. Cauley, III, David P. Stumbo
  • Patent number: 11866766
    Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
    Type: Grant
    Filed: October 21, 2022
    Date of Patent: January 9, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, John Stark, Yadwinder Deol, Vishal Sharma
  • Patent number: 11866771
    Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
    Type: Grant
    Filed: June 11, 2021
    Date of Patent: January 9, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Amy L. Hiddessen, Benjamin J. Hindson
  • Patent number: 11857968
    Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.
    Type: Grant
    Filed: June 24, 2021
    Date of Patent: January 2, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Lung-Hsin Hung, Robert Meltzer, Lucas Frenz
  • Patent number: 11857974
    Abstract: Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.
    Type: Grant
    Filed: March 24, 2021
    Date of Patent: January 2, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Darren R. Link
  • Patent number: 11845980
    Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.
    Type: Grant
    Filed: February 4, 2022
    Date of Patent: December 19, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yi Xue, Joshua Ritchey, Robert Meltzer
  • Patent number: 11841350
    Abstract: A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.
    Type: Grant
    Filed: March 15, 2022
    Date of Patent: December 12, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Christopher Belisle, Mark A. Snyder