Patents Assigned to Bio-Rad Laboratories
  • Methods for processing nucleic acid samples
    Patent number: 10954558
    Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: March 23, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Arkadiusz Bibillo, Pranav Patel
  • Methods for processing nucleic acid samples
    Patent number: 10954557
    Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: March 23, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Arkadiusz Bibillo, Pranav Patel
  • Nucleic acid modifying enzymes
    Patent number: 10954495
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: March 23, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Yan Wang
  • Protein quantitation device
    Patent number: 10948403
    Abstract: Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.
    Type: Grant
    Filed: October 4, 2018
    Date of Patent: March 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Evan Thrush, Steven Swihart, William Strong, Trey Marlowe, Li Lu
  • System and method for leakage control in a particle capture system
    Patent number: 10947581
    Abstract: A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.
    Type: Grant
    Filed: March 17, 2020
    Date of Patent: March 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, John Stark, Yadwinder Deol, Vishal Sharma
  • Universal hairpin primers
    Patent number: 10941445
    Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: March 9, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tal Raz, Pascaline Mary
  • Combination treponemal and non-treponemal syphilis test
    Patent number: 10942182
    Abstract: Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.
    Type: Grant
    Filed: March 27, 2020
    Date of Patent: March 9, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ravi Kaul, Weiming Zheng, Roger Walker
  • Protein-nanoparticle conjugate purification methods
    Patent number: 10934366
    Abstract: Methods and kits for purifying protein-nanoparticle conjugates are provided. In some embodiments, a multimodal medium having a size exclusion mode and a capture mode is used to purify the protein-nanoparticle conjugates.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: March 2, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jiali Liao, Tom Berkelman
  • Systems and methods for handling microfluidic droplets
    Patent number: 10927407
    Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: February 23, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Darren Roy Link
  • Cell capture system and method of use
    Patent number: 10921237
    Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
    Type: Grant
    Filed: August 28, 2020
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Kalyan Handique
  • Recombinant deamidated gliadin antigen
    Patent number: 10921315
    Abstract: Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Roger Walker, Yabin Lu, Urvee Desai, Daming Shan
  • Enhanced purification of antibodies and antibody fragments by apatite chromatography
    Patent number: 10919930
    Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Modification of surface properties of microfluidic devices
    Patent number: 10919038
    Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Joshua Ritchey, Yi Xue, Robert Meltzer
  • Split-cycle and tape amplification
    Patent number: 10920270
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.
    Type: Grant
    Filed: March 22, 2019
    Date of Patent: February 16, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Nicholas Heredia, Dianna Maar
  • System and method for retrieving and analyzing particles
    Patent number: 10914672
    Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
    Type: Grant
    Filed: September 18, 2020
    Date of Patent: February 9, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Austin Payne, Vishal Sharma, Kyle Gleason, Priyadarshini Gogoi, Karthik Ganesan, Brian Boniface, Will Chow
  • Spherical porous hydroxyapatite sorbent and methods thereof
    Patent number: 10914712
    Abstract: Highly spherical sorbents of porous hydroxyapatite materials and methods of producing these sorbents are disclosed. The sorbents of the present invention have good mechanical stability and are useful as chromatography media for the separation of biomolecules, such as proteins and nucleic acids.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: February 9, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: James W. Xu
  • DEVICE FOR GENERATING DROPLETS
    Publication number: 20210031200
    Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
    Type: Application
    Filed: August 17, 2020
    Publication date: February 4, 2021
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. NESS, Christopher F. KELLY, Donald A. MASQUELIER
  • Transposase-based genomic analysis
    Patent number: 10907205
    Abstract: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: February 2, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jennifer Chew
  • System and method for automated single cell processing
    Patent number: 10900032
    Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.
    Type: Grant
    Filed: May 5, 2020
    Date of Patent: January 26, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, William T. Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
  • Modular automated chromatography system
    Patent number: 10900938
    Abstract: Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform may recognize the modules and their locations, coordinate fluid connections between the modules, and provide a variety of control, monitoring, data generating and data processing functions to generate chromatographic data. The software platform may also provide graphical tools for designing chromatographic methods from a library of phases.
    Type: Grant
    Filed: October 8, 2019
    Date of Patent: January 26, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Robert Iovanni, Alec Gordon, Bob Avarbock, Wayne Bland, Glenn Price, Ken Baker, Farah Mavandadi, Christof Schultz