Abstract: Alcohol is manufactured from cellulosic materials by a one-step process involving the simultaneous reaction of a cellulosic material, a cellulase and an alcohol-producing microorganism.

Abstract: In the enzymatic hydrolysis of cellulose to obtain water-soluble sugars, the enzyme source is the aqueous culture mass obtained in an enzyme preparation step by cultivating in an aqueous nutrient medium in the presence of a cellulosic material a cellulolytic microorganism capable of elaborating a cellulolytic enzyme complex which can degrade native cellulose. No separation is made of any component of the culture mass. Such use of the culture mass as the enzyme source not only eliminates processing steps to separate the enzyme, but also results in increased hydrolysis rates and yields of the desired water-soluble sugars in the hydrolysis of cellulose.