Abstract: Improved production of ascorbic acid is obtained empolying Chlorella as a microorganism source and growing the culture under a controlled pattern of carbon source supply. Greatly improved ratios of ascorbic acid to total carbon supplied as well as enhanced ascorbic acid concentrations in the fermentor are obtained.C. pyrenoidosa UV101-158 was deposited at the A.T.C.C. on June 27, 1985 and given Accession No. 53170.