Patents Assigned to BIO 101
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Publication number: 20040132082Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.Type: ApplicationFiled: December 17, 2003Publication date: July 8, 2004Applicant: BIO101Inventors: James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
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Patent number: 6706498Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.Type: GrantFiled: May 22, 2001Date of Patent: March 16, 2004Assignee: Bio101, Inc.Inventors: James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
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Publication number: 20040039188Abstract: The present invention contemplates a system for rapidly isolating nucleic acids. The system comprises an insoluble silica matrix and a buffered aqueous salt solution containing salt at a concentration of at least 3 molar and a buffering agent at a concentration sufficient to provide a buffering capacity corresponding to that which either tris(hydroxymethyl)aminomethane or phosphate ion at a concentration of 0.1 to 1 molar would provide in the solution. Methods of using the system are also contemplated.Type: ApplicationFiled: August 28, 2003Publication date: February 26, 2004Applicant: BIO 101Inventors: James Gautsch, Mark Brolaski
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Patent number: 6613895Abstract: The present invention contemplates a system for rapidly isolating nucleic acids. The system comprises an insoluble silica matrix and a buffered aqueous salt solution containing salt at a concentration of at least 3 molar and a buffering agent at a concentration sufficient to provide a buffering capacity corresponding to that which either tris(hydroxymethyl)aminomethane or phosphate ion at a concentration of 0.1 to 1 molar would provide in the solution. Methods of using the system are also contemplated.Type: GrantFiled: February 22, 2000Date of Patent: September 2, 2003Assignee: BIO 101Inventors: James Gautsch, Mark Brolaski
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Publication number: 20020172949Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.Type: ApplicationFiled: May 22, 2001Publication date: November 21, 2002Applicant: BIO 101, Inc.Inventors: James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
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Patent number: 6235501Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.Type: GrantFiled: September 25, 1997Date of Patent: May 22, 2001Assignee: Bio101, Inc.Inventors: James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
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Patent number: 5658790Abstract: The present invention contemplates a system and formulations for preparing cell culture medium useful for growing cells for the purpose of producing and isolating nucleic acids. Dry-concentrate culture medium compositions are described as packaged in unit dose form such as in dissolvable capsules.Type: GrantFiled: January 25, 1996Date of Patent: August 19, 1997Assignee: Bio 101, Inc.Inventor: James W. Gautsch
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Patent number: 5223225Abstract: An improved "reference" pipet tip supports a number of volume scale ranges on its external body--preferably a scale for each of small volumes 0.5 microliters through 5 microliters, medium volumes 5 microliters to 50 microliters, and large volumes of 50, 100, and 150 microliters--because the geometry of its interior reservoir is non-uniform. In particular, the pipet tip's interior reservoir increases in diameter, and in contained volume, at a first rate, and in response to a first conical angle of rotation, at its distal end, tip, region. The interior reservoir increases at a different, larger, rate at its proximal end region, which proximal end region is connected to a pipettor. Fluid volumes in a broad range from. 0.5 microliters to 150 microliters are typically measurable at accuracies of better than 10%.Type: GrantFiled: May 17, 1991Date of Patent: June 29, 1993Assignee: BIO 101Inventor: Jim Gautsch