Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Type:
Grant
Filed:
May 22, 2001
Date of Patent:
March 16, 2004
Assignee:
Bio101, Inc.
Inventors:
James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Type:
Application
Filed:
May 22, 2001
Publication date:
November 21, 2002
Applicant:
BIO 101, Inc.
Inventors:
James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
Abstract: The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Type:
Grant
Filed:
September 25, 1997
Date of Patent:
May 22, 2001
Assignee:
Bio101, Inc.
Inventors:
James W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
Abstract: The present invention contemplates a system and formulations for preparing cell culture medium useful for growing cells for the purpose of producing and isolating nucleic acids. Dry-concentrate culture medium compositions are described as packaged in unit dose form such as in dissolvable capsules.