Abstract: Collagen-based tissue matrices are treated in a reactor by a flow of supercritical carbon dioxide, including at least one chemical additive inserted via an insertion device that is in communication with a loop. The additive is injected into the liquid or supercritical carbon dioxide when the reactor is already pressurized. The method combines supercritical CO2 as a solvent and a chemical additive as a co-solvent, in a dynamic treatment flow which circulates in the loop associated with the reactor, in order to increase their action on the treated tissue. The circulation of an additive, which is progressively injected and then recirculated with the CO2, forms part of a first treatment cycle. Several cycles, each with an additive, may follow one after the other, separated by a step of separating out the additive and the CO2, which is carried out with or without maintaining the pressurization and the circulation of CO2.

Abstract: The invention relates to a method for preparing an osteoconductive bone paste by heating an initial mixture containing an aqueous solution and a granular material including collagen and minerals. A portion of the collagen and the minerals are contained in grains of bone matrix. The initial mixture is heated enough to transform a portion of the collagen into gelatine and thus to obtain a cohesive bone paste having a predefined viscosity.

Abstract: The invention relates to a method for preparing an osteoconductive bone paste by heating an initial mixture containing an aqueous solution and a granular material including collagen and minerals. A portion of the collagen and the minerals are contained in grains of bone matrix. The initial mixture is heated enough to transform a portion of the collagen into gelatine and thus to obtain a cohesive bone paste having a predefined viscosity.