Patents Assigned to BIOCARTIS NV
  • Publication number: 20210246499
    Abstract: The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.
    Type: Application
    Filed: April 26, 2021
    Publication date: August 12, 2021
    Applicant: Biocartis NV
    Inventors: Bart Claes, Rudi Rossau
  • Patent number: 11028431
    Abstract: The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: June 8, 2021
    Assignee: BIOCARTIS, NV
    Inventors: Bart Claes, Rudi Rossau
  • Patent number: 10829758
    Abstract: The present invention relates to a highly automatable method for isolation and/or purification of nucleic acids from a biological sample, which is particularly suitable for nucleic acids-shorter than 250 bp and can be performed without a proteolytic pre-digestion step in an automated system, preferably a cartridge-based system. In a further aspect, the present invention also provides automated nucleic acid detection methods based on said isolation and/or purification method, as well as buffers and kits to be used in performing said methods.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: November 10, 2020
    Assignee: BIOCARTIS, NV
    Inventors: Geert Meersseman, Klaas DeCanniere
  • Publication number: 20200199653
    Abstract: The present invention is based on the discovery of sensitive and specific methylation detection by a) controlling excessive DNA degradation prior to conversion by incubating DNA conversion reagent (e.g. bisulfite reagent) directly with a nucleic acid containing sample without requiring prior nucleic acid purification from the sample and without requiring prior nucleic acid denaturation at elevated temperatures of 98° C. and, b) optimizing bisulfite removal by controlling the pumping rate flow of the bisulfite treated sample over an extraction membrane inside an automated system.
    Type: Application
    Filed: December 3, 2019
    Publication date: June 25, 2020
    Applicant: Biocartis NV
    Inventors: David Nauwelaers, Hannah Kenens
  • Patent number: 10533210
    Abstract: The present invention is based on the discovery of sensitive and specific methylation detection by a) controlling excessive DNA degradation prior to conversion by incubating DNA conversion reagent (e.g. bisulfite reagent) directly with a nucleic acid containing sample without requiring prior nucleic acid purification from the sample and without requiring prior nucleic acid denaturation at elevated temperatures of 98° C. and, b) optimizing bisulfite removal by controlling the pumping rate flow of the bisulfite treated sample over an extraction membrane inside an automated system.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: January 14, 2020
    Assignee: Biocartis NV
    Inventors: David Nauwelaers, Hannah Kenens
  • Patent number: 10279343
    Abstract: A transfer device for the contamination free transfer of biological material from a sealed chamber to a recipient. The transfer device includes a support having a first docking area and a second docking area. The first docking area has at least one inlet port, each inlet port creating a fluid connection with one sealed chamber. The second docking area has at least one outlet port, each outlet port creating a fluid connection with a recipient. The first docking area further includes a piercing element designed for piercing a sealing element of a sealed chamber in order to allow, together with at least one inlet port, a fluid connection between a pierced sealed chamber and the inlet port, to allow a contamination free transfer of the biological material from the pierced sealed chamber to at least one recipient.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: May 7, 2019
    Assignee: Biocartis NV
    Inventors: Nicolas Vergauwe, Thierry Tollenboom
  • Publication number: 20180371537
    Abstract: The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.
    Type: Application
    Filed: September 22, 2016
    Publication date: December 27, 2018
    Applicant: Biocartis NV
    Inventors: Bart Claes, Rudi Rossau
  • Publication number: 20180311670
    Abstract: A device for sealing liquids inside a selected zone of a fluidic path that runs in a fluidic assembly and then for cutting or removing the thus sealed zone in a spillage- and contamination-free manner from said fluidic assembly. Methods of performing such contamination- and leakage-free sealing and cutting of selected fluidic path zones comprising a liquid of interest from the remaining parts of fluidic assemblies, preferably in a fully automated manner.
    Type: Application
    Filed: November 30, 2016
    Publication date: November 1, 2018
    Applicant: Biocartis NV
    Inventors: Nicolas Vergauwe, Frederik Doucet, Thierry Tollenboom
  • Publication number: 20180291367
    Abstract: The present invention relates to a highly automatable method for isolation and/or purification of nucleic acids from a biological sample, which is particularly suitable for nucleic acids-shorter than 250 bp and can be performed without a proteolytic pre-digestion step in an automated system, preferably a cartridge-based system. In a further aspect, the present invention also provides automated nucleic acid detection methods based on said isolation and/or purification method, as well as buffers and kits to be used in performing said methods.
    Type: Application
    Filed: June 9, 2016
    Publication date: October 11, 2018
    Applicant: Biocartis NV
    Inventors: Geert Meersseman, Klaas DeCanniere
  • Publication number: 20180245131
    Abstract: The present invention generally concerns an automated system capable of performing quantitative PCR (qPCR) analysis of a nucleic acid present in a biological sample together with preparation of a sequencing-ready nucleic acid library from said sample, either simultaneously or sequentially. In a further aspect, the present invention also provides a method for performing qPCR of a nucleic acid present in a biological sample together with simultaneous of sequential preparation of a sequencing-ready nucleic acid library from said sample. Finally, the present invention also provides removable cartridges for use in the automated systems and methods according to the invention.
    Type: Application
    Filed: July 19, 2016
    Publication date: August 30, 2018
    Applicant: Biocartis, NV
    Inventors: Geert MEERSSEMAN, Nicolas VERGAUWE
  • Publication number: 20180243719
    Abstract: The present invention generally-relates to cartridges for automated systems and automated methods for fully automated processing of biological samples to next-generation sequencing-ready nucleic acid libraries. In particular, the present invention concerns a fluidic cartridge comprising a compartment for receiving a biological sample, means for liberating and/or purifying nucleic acids from the received sample and for transporting said nucleic acids to a compartment wherein NGS library can be prepared from said nucleic acids using provided therein reagents. In a particular aspect, the present invention also concerns cartridges for automated systems and automated methods for preparing an NGS nucleic acid library from a biological sample simultaneously or sequentially with a qPCR assay performed on the same biological sample.
    Type: Application
    Filed: July 19, 2016
    Publication date: August 30, 2018
    Applicant: Biocartis, NV
    Inventors: Geert MEERSSEMAN, Nicolas VERGAUWE
  • Publication number: 20180201973
    Abstract: The present invention is based on the discovery of sensitive and specific methylation detection by a) controlling excessive DNA degradation prior to conversion by incubating DNA conversion reagent (e.g. bisulfite reagent) directly with a nucleic acid containing sample without requiring prior nucleic acid purification from the sample and without requiring prior nucleic acid denaturation at elevated temperatures of 98° C. and, b) optimizing bisulfite removal by controlling the pumping rate flow of the bisulfite treated sample over an extraction membrane inside an automated system.
    Type: Application
    Filed: June 10, 2016
    Publication date: July 19, 2018
    Applicant: Biocartis NV
    Inventors: David Nauwelaers, Hanna Kenens
  • Patent number: 9939075
    Abstract: A fluid control and processing system comprising a cartridge and an instrument for operating the cartridge. The cartridge comprises a cylinder body comprising a cylinder and a fluid port. It further comprises dispensing ports to drive a fluid flow, each dispensing port being positioned to be connectable, when the instrument is operating the cartridge, with at least a fluid port by rotation of the cylinder body. The instrument comprises a piston driver for driving the piston. The system further comprises: reversible fastening means for fastening the piston when the piston driver is rotated with respect to the piston, so that the movement of the piston is controlled by the piston driver; and rotating means comprising a cylinder driver constitutive of the instrument for rotating the cylinder body around the axis A.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: April 10, 2018
    Assignee: BIOCARTIS NV
    Inventors: Gerard Kroon, Ronald De Gier
  • Patent number: 9863006
    Abstract: The present invention discloses methods, kits-of-parts, and devices for the selective lysis of eukaryotic cells in a sample comprising micro-organisms such as bacteria unicellular fungi. The selective lysis is obtained by incubating the sample in an ionic surfactant under alkaline conditions.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: January 9, 2018
    Assignee: Biocartis NV
    Inventors: Irene Dobbelaer, Sieglinde Neerken, Paul Van De Wiel, Bart Van Meerbergen, Roel Penterman
  • Patent number: 9688979
    Abstract: The present invention relates to a method for producing a storable dry composition of biomolecule. First a composition is dispensed on a surface. The composition comprises at least a biomolecule, at least a liquid volatile component, at least a polysaccharide being designed for forming together with the at least a biomolecule and a part of the at least a liquid volatile component a matrix displaying a glass transition temperature Tg. Secondly, at least a part of the liquid volatile component is evaporated by adjusting the temperature of said composition to allow the formation of the matrix. Said evaporation step is initiated at an initial temperature T1, and finished up at a final temperature T2, said final temperature T2 being above said initial temperature T1.
    Type: Grant
    Filed: June 11, 2014
    Date of Patent: June 27, 2017
    Assignee: BIOCARTIS NV
    Inventors: Anke Pierik, Henk Van Damme, Martijn Van Zelst, Rene Bakker, Pieter De Bokx
  • Patent number: 9663813
    Abstract: A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; and (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.
    Type: Grant
    Filed: June 26, 2013
    Date of Patent: May 30, 2017
    Assignee: Biocartis NV
    Inventors: Roel Penterman, Bart Van Meerbergen
  • Publication number: 20170100719
    Abstract: Cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample. The cartridge includes one or more nucleic acid sequences, wherein the cartridge has a generic part and one or more separate application-specific parts, which are connectable to the generic part.
    Type: Application
    Filed: December 21, 2016
    Publication date: April 13, 2017
    Applicant: BIOCARTIS NV
    Inventors: Chris VAN HAAG, Michiel Johannes JONGERIUS, Danny Genius Aldegonda SCHAEFER, Adrianus Wilhelmus Dionisius Maria VAN DEN BIJGAART, Ronald DE GIER, Michiel DE JONG, Gerhard PROSS, Johannes BACHER, Andreas BOOS, Gerd LUEDKE, Jens-Peter SEHER
  • Patent number: 9568424
    Abstract: Cartridge and method for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a one or more nucleic acid sequences, and the cartridge includes a generic part and one or more separate application-specific parts, which are connectable to the generic part.
    Type: Grant
    Filed: June 16, 2006
    Date of Patent: February 14, 2017
    Assignee: BIOCARTIS NV
    Inventors: Chris Van Haag, Michiel Johannes Jongerius, Danny Genius Aldegonda Schaefer, Adrianus Wilhelmus Dionisius Maria Van Den Bijgaart, Ronald De Gier, Michiel De Jong, Gerhard Pross, Johannes Bacher, Andreas Boos, Gerd Luedke, Jens-Peter Seher
  • Patent number: 9522398
    Abstract: System for performing biological analysis comprising: a cartridge having a deformable chamber suitable for containing a liquid to be analyzed; a heater having a heating face arranged to heat the liquid contained in the deformable chamber; characterized in that the heating face of the heater is moveable from a first position to at least a second position, different from the first position, to allow an increase of volume of said deformable chamber in order to compensate an increase of pressure in the deformable chamber during the heating of said liquid by said heater.
    Type: Grant
    Filed: June 27, 2013
    Date of Patent: December 20, 2016
    Assignee: BIOCARTIS NV
    Inventors: Bart Van Meerbergen, Roel Penterman, Nicolas Vergauwe
  • Patent number: D851510
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: June 18, 2019
    Assignee: BIOCARTIS NV
    Inventor: Caroline Jans