Patents Assigned to Biocept, Inc.
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Publication number: 20240002905Abstract: The present disclosure provides pathogen inactivating formulations, and methods of use thereof in collecting, transporting and storing biological samples, as well as preventing degradation of and purifying nucleic acids. The disclosure further provides methods of preparing compositions comprising the pathogen inactivating formulation and a biological sample.Type: ApplicationFiled: November 11, 2021Publication date: January 4, 2024Applicant: Biocept, Inc.Inventor: Lyle ARNOLD
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Patent number: 11719692Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: GrantFiled: November 26, 2019Date of Patent: August 8, 2023Assignee: BIOCEPT, INC.Inventors: Stephen D. Mikolajczyk, Tony Pircher, Pavel Tsinberg, Farideh Z. Bischoff
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Patent number: 10745749Abstract: The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.Type: GrantFiled: October 30, 2017Date of Patent: August 18, 2020Assignees: Biocept, Inc., Aegea BiotechnologiesInventor: Lyle Arnold
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Patent number: 10527611Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: GrantFiled: July 29, 2015Date of Patent: January 7, 2020Assignee: Biocept, Inc.Inventors: Stephen Mikolajczyk, Tony Pircher, Pavel Tsinberg, Farideh Z. Bischoff
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Patent number: 10369568Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.Type: GrantFiled: June 27, 2016Date of Patent: August 6, 2019Assignee: BIOCEPT, INC.Inventors: Zhongliang Tang, Ram Bhatt, Pavel Tsinberg
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Patent number: 9834817Abstract: The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.Type: GrantFiled: March 15, 2013Date of Patent: December 5, 2017Assignees: AEGEA BIOTECHNOLOGIES, BIOCEPT, INC.Inventor: Lyle Arnold
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Patent number: 9671407Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: GrantFiled: March 24, 2014Date of Patent: June 6, 2017Assignee: BIOCEPT, INC.Inventors: Stephen D. Mikolajczyk, Tony Pircher, Farideh Z. Bischoff, Pavel Tsinberg
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Patent number: 9347946Abstract: The present invention provides reagents containing binding moieties conjugated to dextran moieties, methods of making such reagents, and use of such reagents in a variety of molecular and cellular assays.Type: GrantFiled: September 22, 2011Date of Patent: May 24, 2016Assignee: BIOCEPT, INC.Inventors: Stephen D. Mikolajczyk, Lisa S. Millar
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Patent number: 9212977Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.Type: GrantFiled: June 15, 2012Date of Patent: December 15, 2015Assignee: BIOCEPT, INC.Inventors: Zhongliang Tang, Pavel Tsinberg, Ram S. Bhatt
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Patent number: 9128082Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: GrantFiled: March 24, 2010Date of Patent: September 8, 2015Assignee: BIOCEPT, INC.Inventors: Stephen Mikolajczyk, Tony Pircher, Pavel Tsinberg, Farideh Z. Bischoff
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Patent number: 9040255Abstract: The present invention provides methods for preventing clumping of cells in microfluidic devices by addition of diazolidinyl urea (DU). DU can be added to samples at the time of collection or can be added to samples post-collection. DU can also be pre-added to sample collection devices.Type: GrantFiled: September 23, 2011Date of Patent: May 26, 2015Assignee: BIOCEPT, INC.Inventors: Pavel Tsinberg, Stephen D. Mikolajczyk
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Publication number: 20150056614Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: ApplicationFiled: March 24, 2014Publication date: February 26, 2015Applicant: Biocept, Inc.Inventors: Stephen D. MIKOLAJCZYK, Tony PIRCHER, Farideh Z. BISCHOFF, Pavel TSINBERG
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Publication number: 20140335514Abstract: The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.Type: ApplicationFiled: March 15, 2013Publication date: November 13, 2014Applicants: AEGEA BIOTECHNOLOGIES, BIOCEPT, INC.Inventor: Lyle ARNOLD
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Publication number: 20130121895Abstract: A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.Type: ApplicationFiled: June 15, 2012Publication date: May 16, 2013Applicant: BIOCEPT, INC.Inventors: Zhongliang TANG, Pavel TSINBERG, Ram S. BHATT
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Publication number: 20120258475Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.Type: ApplicationFiled: April 6, 2012Publication date: October 11, 2012Applicant: BIOCEPT, INC.Inventors: Zhongliang TANG, Ram S. BHATT, Pavel TSINBERG
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Publication number: 20120164661Abstract: The present invention provides reagents containing binding moieties conjugated to dextran moieties, methods of making such reagents, and use of such reagents in a variety of molecular and cellular assays.Type: ApplicationFiled: September 22, 2011Publication date: June 28, 2012Applicant: BIOCEPT, INC.Inventors: Stephen D. MIKOLAJCZYK, Lisa S. MILLAR
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Publication number: 20120164676Abstract: The present invention provides methods for preventing clumping of cells in microfluidic devices by addition of diazolidinyl urea (DU). DU can be added to samples at the time of collection or can be added to samples post-collection. DU can also be pre-added to sample collection devices.Type: ApplicationFiled: September 23, 2011Publication date: June 28, 2012Applicant: BIOCEPT, INC.Inventors: Pavel TSINBERG, Stephen D. MIKOLAJCZYK
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Publication number: 20120100538Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.Type: ApplicationFiled: October 7, 2011Publication date: April 26, 2012Applicant: Biocept, Inc.Inventors: Stephen D. Mikolajczyk, Tony Pircher, Farideh Z. Bischoff, Pavel Tsinberg
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Patent number: 8158410Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.Type: GrantFiled: January 18, 2005Date of Patent: April 17, 2012Assignee: Biocept, Inc.Inventors: Zhongliang Tang, Ram S. Bhatt, Pavel Tsinberg
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Patent number: 8076127Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.Type: GrantFiled: January 18, 2005Date of Patent: December 13, 2011Assignee: Biocept, Inc.Inventors: Zhongliang Tang, Ram S. Bhatt, Pavel Tsinberg