Abstract: The present invention relates to a method for prenatal diagnosis using digital PCR, and more particularly to a method for providing information for diagnosis of chromosomal aneuploidy in a fetus, comprising: (a) extracting DNAs from pregnant woman's blood; (b) classifying the DNAs according to size to obtain DNAs having a size of 1,000 bp or less; (c) performing digital PCR using the obtained DNAs of step (b), for a control gene located on a chromosome not associated with chromosomal aneuploidy and a target gene located on a chromosome associated with chromosomal aneuploidy; (d) calculating a ratio of a quantitative digital PCR value of the target gene to a quantitative digital PCR value of the control gene; and (e) determining that when the ratio calculated in step (d) is 0.70-1.14, a chromosome number of the fetus is normal.
Type:
Grant
Filed:
October 28, 2016
Date of Patent:
October 18, 2022
Assignee:
BIOCORE CO., LTD.
Inventors:
Seung Yong Hwang, Moon Ju Oh, Seung Jun Kim, Jong Pil Youn, Ji Hoon Kim, Seung Yong Lee, Jeong Jin Ahn, Joon Soo Park, Hyo Jung Choi
Abstract: The present invention discloses a method for analyzing a HCV genotype by extracting RNA from plasma and serum, performing C-type hepatitis virus (HCV) RT-PCR and then making them react on an oligonucleotide chip. The present invention also provides a method for simply and exactly examining the analysis of the HCV genotype for four people on one slide.
Type:
Grant
Filed:
April 10, 2002
Date of Patent:
February 20, 2007
Assignee:
Biocore Co., Ltd.
Inventors:
Young-Suk Park, Jae-Chan Park, Eun-Ha Kim