Abstract: The determination of the single enzymatic activities of the cytoplasmatic and mitochondrial isoenzymes of Glutamic-Oxalacetic Transaminase in samples of serum or plasma is effected by determining the GOT activity before and after incubation with L-serine-O-sulphate.
Abstract: A kinetic UV method of determining GOT and GPT by reacting transaminase with a substrate uses L-cysteinsulfinic acid and L-alanine as the substrate and lactic dehydrogenase as the revealing enzyme.